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This quasi experimental research aimed to study the effectiveness of Aedes aegypti larva controlled by leaf essential oils from Eucalyptus globulus Labill. Completely randomized design (CRD) was conducted. The area of Thatphanom Crownprince Hospital was selected to put the experimental containers. Fifteen of experimental containers each group were used. The 1 ml of leaf essential oils from Eucalyptus globulus Labill was used with experimental group. The duration of the experimental period was 14 days. The mosquito larva was assessed every 2 days. Descriptive statistic was used for data analysis. The results found that the control group found 1 jar with the mosquito larva at the 8th days, 5 jars with the mosquito larva at the 10th days, 10 jars with the mosquito larva at the 12nd days and 15 jars with the mosquito larva at the 14th days. Whereas the experimental group was not found. The results of this study showed that the leaf essential oil of Eucalyptus globulus Labill might be considered as a potent source for the production of larvicide. Further studies by increasing the concentration and duration of the trial, including using it in the community, should continue.
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