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Tadehagi triquetrum (L.) H. is a medicinal plant that has traditionally been used as an antimicrobial agent for extending the expiration date of products containing water as vehicle. Thai folk medicine practitioners have also used a decoction of T. triquetrum leaves for improving physical and sexual performances in men. The aim of our study was to determine the bioactivity of T. triquetrum extracts using different polar and nonpolar solvents. We evaluated the antioxidant activity of extracts using DPPH and ABTS assays. We investigated phosphodiesterase-5 (PDE-5) inhibitory activity of the extracts, which is associated with enhancing sexual and physical performance. We measured antimicrobial activity of the extracts using disc diffusion and minimal inhibitory concentration (MIC). The results revealed that the ethanolic and water extracts showed potent antioxidant and antimicrobial activities. Water extract presented the highest antioxidant activity (IC50 = 12.45 ± 0.14 µg/mL). Polar extracts inhibited the growth of microorganisms S. aureus, E. coli and Ps. Aeruginosa. The non-polar extract effectively suppressed fungal growth. Interestingly, the Thai folk medicine formulation of T. triquetrum water extract at 50 µg/mL inhibited PDE-5 activity by 60.45% (SD = 0.14). This level of inhibition is considered good bioactivity. As these findings are promising, future in vitro, in vivo, and clinical studies should be conducted to examine properties of this plant in greater detail.
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