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This study aimed to investigate the antibacterial efficacy of Artocarpus lakoocha Roxb., heartwood crude extract by maceration, in different solvents against common human pathogens. It was found that A. lakoocha Roxb. crude extract in 95% ethanol showed strong antibacterial activity. The inhibition zone diameter towards Aeromonas hydrophila TISTR 1321, Bacillus cereus TISTR 747, Bacillus licheniformis TISTR 1455, Escherichia coli TISTR 117, Staphylococcus aureus TISTR 746 and Vibrio parahaemolyticus TISTR 1596 were 19.89 ± 0.67, 18.27 ± 0.69, 14.92 ± 0.98, 17.06 ± 0.66, 14.84 ± 0.82 and 19.00 ± 0.42 millimeters, respectively. Yet, the antibacterial activity was not observed when using hexane and water as a solvent. Minimum Bactericidal Concentration, MBC of A. lakoocha Roxb. crude extract in 95% ethanol was in the range of 3,125–25,000 ppm. This data indicated that the extraction solvent plays a vital role in the antibacterial activity of A. lakoocha Roxb. crude extract. A. lakoocha Roxb. crude extract in 95% ethanol was able to destroy both gram positive and negative bacteria, but the efficacy might be different among pathogenic bacteria.
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