Antifungal activity of Rhinacanthus nasutus (L.) Kurz extracts against dermatophytes N/A

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Patamaporn Pruksakorn
Chattraporn Jaima
Parnuphan Panyajai
Nanthawan Mekha
Rinrapas Autthateinchai
Panadda Dhepakson

Abstract

Rhinacanthus nasutus (L.) Kurz (thongphanchang in Thai) has been used in Thai traditional medicine for the treatment of skin diseases and eczema. This plant exhibits the potent antifungal activity against dermatophytes. This study aimed to investigate the antifungal activity of R. nasutus extracts from various parts of the plant and determine the quantity of active substances for developing the extraction method to furnish the antifungal-rich extract. The dried leaves, stems and roots of R. nasutus were extracted with ethanol by maceration and ultrasonic-assisted extraction. The extracts were tested for their antifungal activity using three dermatophytes (Trichophyton rubrum, Trichophyton mentagrophytes, and Microsporum gypseum) by broth microdilution method. Each extract was used for HPLC fingerprint analysis. The root extract showed the most potent antifungal activity against the tested dermatophyte species. Purification and structure elucidation revealed that the active substances are rhinacanthins B, C, N, and Q. In addition, the ethanolic extract of dried root that was further separated with ethyl acetate afforded a rhinacanthins-rich extract. This extract exhibited excellent antifungal activity, comparable to rhinacanthin C (a major active substance). Therefore, this extraction method might be suitable for preparing a R. nasutus root extract in order to develop antifungal herbal formulations.

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Section
Original Articles
Author Biographies

Patamaporn Pruksakorn, N/A

N/A

Chattraporn Jaima, N/A

N/a

Parnuphan Panyajai, N/A

N/A

Nanthawan Mekha, N/A

N/A

Rinrapas Autthateinchai, N/A

N/A

Panadda Dhepakson, N/A

N/A

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