Detection of Coliform Bacteria in Unsealed Drink using the Most Probable Number Technique

Abstract

The objectives of this study were to detect the contamination of coliform bacteria fecal coliform bacteria and Escherichia Coli in unsealed drinks using the Most Probable Number (MPN) method and to find the relationship between contamination of coliform bacteria and contamination of fecal coliform bacteria in unsealed drinks. We collected 35 samples of unsealed drinks from 7 drink stalls at Ongkharak Subdistrict Municipality area. The results found that 28 samples were contaminated with coliform bacteria, representing 80 percent; 23 samples were contaminated with fecal coliform bacteria, representing 65.7 percent; and 19 samples were contaminated with Escherichia coli, representing 54.3 percent. The sample that does not meet the standard with the number of coliform bacteria and fecal coliform bacteria >2.2 MPN/ 100 ml.  It was found that contaminated coliform bacteria and fecal coliform bacteria in unsealed drinks had a statistically significant relationship (p-value<0.05). This study has shown that consumption of unsealed drinks is contaminated with coliform bacteria, fecal coliform bacteria, and Escherichia coli, which are at risk of gastrointestinal infections. Therefore, hygiene and good hygiene procedures must be required to prevent contamination of pathogenic microorganisms. For example, people who handle food should wear gloves, sanitary masks and wash their hands. Shops should also be equipped with equipment to protect animals and insect carriers.