An analysis of Aflatoxin levels in Thai and foreign cigarette fibers
Keywords:
Aflatoxin, Ultrasonic extraction, HPLC, CigaretteAbstract
This research employed an ultrasonic extraction technique to quantify Aflatoxin levels in cigarette samples. Parameters evaluated for optimal extraction conditions included solvent volume, extraction time, solvent type, and suitable solvent ratios. The results indicated that the most effective conditions for Aflatoxin extraction from cigarette samples were achieved using a solvent mixture of acetonitrile and methanol in a 1:1 v/v ratio, with a volume of 60 milliliters, and extraction conducted via ultrasonic equipment for 40 minutes. Analysis of both Thai and international cigarette samples revealed no detectable levels of Aflatoxin in any sample, even after storage for up to 12 months. These findings suggested consumer safety concerning Aflatoxin contamination in cigarettes.
References
Jiang Y, Ogunade IM, Vyas D, Adesogan AT. Aflatoxin in dairy cows: toxicity, occurrence in feedstuffs and milk and dietary mitigation strategies. Toxins. 2021;13:283.
Campos-Avelar I, Colas de la Noue A, Durand N, Cazals G, Martinez V, Strub C, Fontana A, Schorr-Galindo S. Aspergillus flavus growth inhibition and aflatoxin b1 decontamination by streptomyces isolates and their metabolites. Toxins. 2021;13:340.
Stratton J, Shiplo S, Ward M, Babayan A, Stevens A, Edwards S. Assessing contraband tobacco in two jurisdictions: a direct collection of cigarette butts. BMC Public Health. 2016;16:622.
Areej Zuhair A, Mrwa Thamer H, Maha Muhamed K, Adel Saadi ALS, Noor Ibrahim K. Determination of Aflatoxins in feed by HPLC and PCR. J Food Pharm Sci. 2020;8:315-23.
Kim HJ, Lee MJ, Kim HJ, Cho SK, Park HJ, Jeong MH. Analytical method development and monitoring of Aflatoxin B1, B2, G1, G2 and Ochratoxin A in animal feed using HPLC with fluorescence detector and photochemical reaction device. Cogent Food Agriculture. 2017;3:1419788.
Hetmanski MT, Scudamore KA. A simple quantitative HPLC method for determination of Aflatoxins in cereals and animal feedstuffs using gel permeation chromatography clean‐up. Food Additives Contaminants. 1989;6:35-48.
Gazioğlu I, Kolak U. Method validation for the quantitative analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in processed cereal-based foods by HPLC with fluorescence
detection. J AOAC Int. 2015;98:939-45.
Gholizadeh S, Mirzaei H, Khandaghi J, Afshar Mogaddam MR, Javadi A. Ultrasound–assisted solvent extraction combined with magnetic ionic liquid based-dispersive liquid–liquid microextraction for the extraction of mycotoxins from tea samples. J Food Comp Analysis. 2022;114:104831.
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