VEGF 936 C/T polymorphisms in Thai patients with hepatocellular carcinoma
Keywords:
Hepatocellular carcinoma, Gene polymorphism, VEGF gene, PrognosisAbstract
Introduction Vascular endothelial growth factor (VEGF) genes play an important role in
angiogenesis and carcinogenesis of several cancers, including hepatocellular carcinoma (HCC).
Several studies have reported that VEGF 936 C/T gene polymorphisms are associated with risk
and prognosis in cancer patients.
Objective To investigate the relationships between VEGF 936 C/T polymorphisms and
hepatocellular carcinoma risk (HCC), clinico-pathological parameters, as well as the overall
survival of patients with hepatocellular carcinoma in Thailand.
Material and Methods DNA samples were extracted from 82 paraffin embedded tissues with
HCC and 253 peripheral blood samples from healthy subjects as a control group. VEGF 936 C/T
gene polymorphisms were determined using polymerase chain reaction-restriction fragment
length polymorphism (PCR –RFLP).
Results The frequencies of CC, CT and TT genotypes were 69.6%, 28.9% and 1.6% respectively
in the healthy control group. The HCC patient group exhibited CC and CT genotype frequencies
at 67.1% and 32.9% respectively, with no TT genotype frequency. Furthermore, the frequency
of C and T alleles were 84.0 and 16.0 in the healthy control group, and 83.5% and 16.5%
respectively in the HCC patient group.
Conclusions Our study showed no association found between VEGF 936 C/T polymorphisms
and HCC risk, (P=0.43), clinico-pathological parameters, as well as the overall survival of the
patients (P = 0.40).
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