Development of Practice Guideline for Appropriate Laryngoscope Cleaning to Reduce Bacterial Contamination in Laryngoscopes used in Roi Et Hospital

Abstract

Purposes : To development the guidelines for cleaning laryngoscope to reduce bacterial contamination and to study the results of applying the guideline and the satisfaction of nursing personnel in Roi Et Hospital.
Design : Research and Development
Materials and Methods : was developed using the evidence-based practice model of    Soukup (2000) as a conceptual framework. An evidence-based practice model had four phases which  consisted of 1) Evidence triggered phase, 2)  Evidence supported  phase, 3)  Evidence observed phase,  and 4)Evidence-based phase. The study sites of this study were Anesthesiology and Internal Medicine (9 wards) of Roi Et Hospital during April 2019 to April 2021. The research instruments were practice guidelines for cleaning laryngoscope and satisfaction questionnaire for practice guidelines used.The descriptive statistics were used for all data analysis.
Main findings : of 50 study subjects all of them were female with mean age 38.9 years (SD=7.56), mean work experience 7.92 years (SD =5.38). The Adenosine Triphosphate (ATP) test, 31 laryngoscope blade culture, 11 handle culture were found ATP was in the standard 100%, positive culture 16% with Bacillus spp. and Coagulase negative staphylococci. The overall satisfaction of practice guidelines used was high level (mean =3.94; SD=0.25).The practice guidelines of laryngoscope cleaning consisted of 6 steps 1) after used release laryngoscope in a plastic bag, fold the mouth of the bag before washing, use hand grab plastic bag to remove the saliva or of contaminants secretion. 2) Lead laryngoscope clean with tap water that flows from the tap. 3) Brush laryngoscope with 4% chlorhexidine scrub or 7.5% povidone iodine scrub. 4) Rinse with clean tap water again and dry. 5) Wipe with 70% alcohol, wait until dry, and keep in a clean carton or plastic bag completely closed. 6) Before used laryngoscope wipe by 70% alcohol to reduce contamination.
Conclusion and recommendations : The laryngoscope cleaning guidelines of this study consisted of six important steps. After applying guideline it was found that the Adenosine Triphosphate (ATP) test result was 100% it was in the standard. The culture found bacteria contamination decrease and overall satisfaction after guideline used it was a high level