Development of Laboratory, Competence, the Study in Microbiological Measurement Uncertainty of Total Plate Count in Ready to Eat Numprik Product and Kanom

Abstract

The development of laboratory, competence accordding to ISO/IEC 17025: 2005,
measurement uncertainty is one of the general requirement that testing and calibration
laboratories should have procedures for estimate uncertainty of measurement. Microorganism,
count is the empirical method that can not show true and bias value, microbiological
measurement uncertainty by “top–down” or “global” approach concept can be used for
calculate from standard deviation of reproducibility (SR). All most in the same laboratory
always used Intralaboratory standard deviation of reproducibility that further used to report
with the analytical report. In 2011, the Regeional Medical Science Center 3 Nakhon Sawan
laboratory had analysed total plate count in food product ready to eat numprik and kanom
33 and 50 samples respectively, and calculated SR of each product , the results show that SR
is ± 0.1462 and± 0.1970 respectively and calculated expanded uncertainty (U) further,
that can be used to report in many patterns of total plate count with the uncertainty of
measurement.