黄芩与黄连提取物对金黄色葡萄球菌的抗菌活性及抗氧化活性研究
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摘要
多种中药材含有具有抗菌活性的化学成分，可对金黄色葡萄球菌（Staphylococcus aureus，S. aureus）产生抑制作用，而该菌为医院获得性感染的重要致病菌之一。本研究旨在探讨黄芩与黄连提取物对甲氧西林敏感金黄色葡萄球菌（methicillin-susceptible S. aureus, MSSA）及甲氧西林耐药金黄色葡萄球菌（methicillin-resistant S. aureus, MRSA）的抗菌活性，同时采用 DPPH 自由基清除法评价其抗氧化活性，并通过 Folin-Ciocalteu 法测定总酚含量。采用比色肉汤微量稀释法测定结果显示，黄芩对 MSSA 的最低抑菌浓度（MIC）及最低杀菌浓度（MBC）分别为 1.56 mg/mL 和 1.56–12.50 mg/mL；对 MRSA 分别为 1.56 mg/mL和 1.56–6.25 mg/mL。黄连表现出更强的抗菌活性，其对 MSSA 的 MIC 及 MBC 分别为 0.39 mg/mL 和 0.78–1.56 mg/mL；对 MRSA 分别为 0.39–0.78 mg/mL 和 0.78–6.25 mg/mL。抗氧化实验结果显示，黄芩与黄连的半数抑制浓度（IC50）分别为 0.067±0.017 mg/mL 和 0.196±0.018 mg/mL。总酚含量测定结果表明，黄芩（44.8 mg GAE/g）高于黄连（36.11 mg GAE/g）。综上所述，两种中药提取物均对 MSSA 及 MRSA 具有良好的抗菌作用，且差异无统计学意义。其中，黄连的抗菌活性优于黄芩，而黄芩的抗氧化能力更强。本研究结果提示，黄芩与黄连均具有作为治疗金黄色葡萄球菌感染或抗氧化干预的潜在应用价值。
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参考
Department of Disease Control, Ministry of Public Health. Antimicrobial resistance surveillance and investigation guidelines. 1st ed. Bangkok: Canna Graphic; 2022. (in Thai)
Wielders CL, Fluit AC, Brisse S, Verhoef J, Schmitz FJ. mecA gene is widely disseminated in Staphylococcus aureus population. J Clin Microbiol. 2002;40(11):3970-5.
World Health Organization. WHO bacterial priority pathogens list, 2024: bacterial pathogens of public health importance to guide research, development and strategies to prevent and control antimicrobial resistance. Geneva: World Health Organization; 2024.
Kim G, Gan RY, Zhang D, Farha AK, Habimana O, Mavumengwana V, et al. Large-scale screening of 239 traditional Chinese medicinal plant extracts for their antibacterial activities against multidrug-resistant Staphylococcus aureus and cytotoxic activities. Pathogens. 2020;9(3):185.
Leach FS. Anti-microbial properties of Scutellaria baicalensis and Coptis chinensis, two traditional Chinese medicines. Bioscience Horizons. 2011;4(2):119-27.
Chanchal DK, Singh K, Bhushan B, Chaudhary JS, Kumar S, Varma AK, et al. An updated review of Chinese skullcap (Scutellaria baicalensis): emphasis on phytochemical constituents and pharmacological attributes. Pharmacological Research - Modern Chinese Medicine. 2023;9:100326.
Xu M, Ma Y, Wan LY. Antibacterial and anti-inflammatory potential of Chinese medicinal herbs: Lonicerae flos, Lonicerae japonicae flos, Scutellaria baicalensis Georgi, and Forsythia suspensa. Nat Prod Commun. 2022;17(11):1-21.
Powtongsook W, Rangsipanuratn W, Tubchan W, Semuang P, Sutin S, Meesin S. Antioxidant, antibacterial and anti-biofilm activities of three Chinese herbs against methicillin-resistant Staphylococcus aureus. HCU Journal. 2023;27(1):78-90. (in Thai)
Chulsomlee S, Sinthupibulyakit C, Moolthiya P, Pirat N, Vichittumaros K, Narasinviwat N, et al. Total phenolic and flavonoid compounds of Chinese herbs extracts: Atractylodes lancea (Thunb.) DC., Atractylodes macrocephala Koidz and Coptis chinensis Franch. Journal of Health Sciences and Wellness. 2025;29(1):116-25. (in Thai)
He QQ, Meneely J, Grant IR, Chin J, Fanning S, Situ C. Phytotherapeutic potential against MRSA: mechanisms, synergy, and therapeutic prospects. Chin Med. 2024;19(1):89.
Kim EH, Jang YA, Kim SB, Kim HH, Lee JT. Antimicrobial, antifungal effect and safety verification using BCOP assay of extracts from Coptis chinensis. J Appl Biol Chem. 2018;61(3):297-304.
Gongmeng U, Powtongsook W, Sutin S, Sillamut O. Antimicrobial and antioxidant activities of Chinese herb extracts against skin pathogenic microorganisms. Proceedings 12th National & International Conference; 2021 Jan 15; Bangkok: Graduate School, Suan Sunandha Rajabhat University; 2021. (in Thai)
Porwal P, Sharma SK. Synergistic effect between vancomycin and traditional Chinese medicine (TCM) herbs against methicillin-resistant Staphylococcus aureus (MRSA) infections. Pharmacological Research - Modern Chinese Medicine. 2024;13:100538.
Lee KW, Park SI. Anti-oxidant, anti-bacterial, and anti-inflammatory activity of Scutellaria baicalensis root supercritical fluid extracts. J Anim Plant Sci. 2024;34(6):1410-23.
Lim J, Kim K, Kwon DY, Kim JK, Sathasivam R, Park SU. Effects of different solvents on the extraction of phenolic and flavonoid compounds, and antioxidant activities, in Scutellaria baicalensis hairy roots. Horticulturae. 2024;10(2):160.
Liau PR, Wu MS, Lee CK. Inhibitory effects of Scutellaria baicalensis root extract on linoleic acid hydroperoxide-induced lung mitochondrial lipid peroxidation and antioxidant activities. Molecules. 2019;24(11):2143.
