Tanshinones encapsulated PLGA nanoparticles: preparation and the effect on cognitive improvement with the combination of borneol
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Abstract
The cognitive impairment belongs to the most comment symptom caused by several central nervous system (CNS) diseases, leading to the lowered living quality. However, these commonly therapeutic agents scarcely exhibit the satisfactory clinical outcome, due to the unclear pathogenesis as well as the delivery challenges posed by blood-brain barrier. The purpose in this study is to develop a therapeutic approach with the combination of tanshinones co-loaded nanoparticles (NPs) and borneol, to improve the cognitive impairment. Primarily, tanshinone IIA (TanIIA) and cryptotanshinone (CTan), which are the main tanshinones derived from traditional Chinese herbal Salvia miltiorrhiza Bunge, were loaded into PLAG copolymer (TC@PLGA/NPs) by one-step solvent evaporation method. TC@PLGA/NPs exhibited the encapsulation efficiency of TanIIA and CTan were 85.31±1.28% and 86.42±2.07%, respectively. LE% of TanIIA and CTan were 1.24±0.09% and 1.53±0.15%, respectively. Meanwhile, it had the average particle size of 194.2±3.5 nm and good storage stability. The DSC results indicated that the tanshinones were well encapsulated in PLGA polymer, instead of the physical mixture. The Morris water maze (MWM) behavioral experiment demonstrated that TC@PLGA/NPs with the combination of borneol (BNL+TC@PLGA/NPs) could dramatically improve the spatial learning and memory capability of AD model rats, compared with other groups. Specifically, the administration of BNL+TC@PLGA/NPs can significantly shorten the escape latency and swimming distance of scopolamine-induced dementia rats, meanwhile it can also reduce the expression of MDA and AchE in hippocampus. In conclusion, combined the advantage of borneol to enhance drug delivery across BBB and the tanshinones co-loaded NPs, the learning ability and cognitive function could be significantly improved. It would provide a useful strategy for CNS diseases treatment.
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References
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