Biodegradation of Phenol in Sediment-Slurry under Aerobic Condition
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Abstract
Biodegradation of phenol in sediment-slurry sample from rice paddy was investigated under aerobic condition in this study. These slurry samples were contaminated with nitrate (0.031 mM), nitrite (0.028 mM) but not with phenol or p-hydroxybenzoate. In active experiment, 0.1 mM of phenol applied at the first time (day 0) and the second time (day 6) was completely biodegraded within 2 days. When 0.3 mM of phenol was reapplied in all treatments, the chemical was completely biodegraded within 5 days of the experiment. The gas production was monitored in the active experiment with 0.1 mM of phenol at the first day of the experiment and then it decreased all along the experiment. Bacillus sp. was isolated and identified from active treatments containing sediment-slurry from rice paddy under aerobic conditions.
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References
บัญญัติ สุขศรีงาม (2534) จุลชีววิทยาทั่วไป. กรุงเทพฯ: โอ. เอส. พริ้นติ้ง เฮ้าส์.
มนัส สุวรรณ (2532) นิเวศวิทยากับการพัฒนาเศรษฐกิจ. กรุงเทพฯ: โอเดียนสโตร์.
สมศักดิ์ วังใน (2528) จุลินทรีย์และกิจกรรมในดิน. กรุงเทพฯ: ไทยวัฒนาพานิชจำ กัด.
สุบัณฑิต นิ่มรัตน์ และสุกานดา เกื้อกิจกูล (2546) การย่อย สลาย p-Hydroxybenzoate โดยใช้ mixed culture ภายใต้สภาวะที่มีออกซิเจน. ใน การประชุมวิชาการ ครั้งที่ 41 มหาวิทยาลัยเกษตรศาสตร์ กรุงเทพฯ วันที่ 3 - 5 กุมภาพันธ์ 2546.
Association of Official American Chemists [AOAC]. (2002). Official methods of analysis. Association of Official American Chemists, MD.
Basha, K.M., Rajendran, A. & Thangavelu, V. (2010). Recent advances in the biodegradation of phenol: A review. Asian Journal of Experimental Biological Sciences, 1 (2), 219 - 234.
Bayly, R.C. & Wigmore, G.J. (1973). Metabolism of phenol and cresols by mutants of Pseudomonas putida. Journal of Bacteriology, 113 (3), 1112 - 1120.
Bertani, I., Kojic, M. & Venturi, V. (2001). Regulation of the p-hydroxybenzoic acid hydroxylase gene (pobA) in plant-growth-promoting Pseudomonas putida WC358. Microbiology, 147, 1611 - 1620.
Cavallo, R.S. & Stabili, L. (2002). Presence of vibrios in seawater and Mytilus alloprovincialis (Lam.) from the Mar Piccolo of Toranto (Ionian Sea). Water Research, 36, 3719 - 3726.
Cerniglia, C.E. (1992). Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation, 3, 351 - 368.
Healy, J.B. & Young, L.Y. (1979). Anaerobic biodegradation of eleven aromatic compounds to methane. Applied and Environmental Microbiology, 38 (1), 84 - 89.
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. & Williams, S.T. (1994). Bergey’s Manual of Systematic Bacteriology, Vol.1. Baltimore: Williams & Wilkins.
Krieg, N.R. & Holt, J.G. (1984). Bergey’s Manual of Systematic Bacteriology, Vol.1. Baltimore: Williams & Wilkins.
Mutzel, A., Reinscheid, U.M., Antranikian, G. & Muller, R. (1996). Isolation and characterization of a thermophilic bacillus strain, that degrades phenol and cresol as sole carbon source at 70 0 C. Applied Microbiol Biotechnol, 46, 593 - 596.
Nair, C.I., Jayachadran, K. & Shashidhar, S. (2008). Biodegradation of phenol. African Journal of Biotechnilogy, 7 (25), 4951 - 4958.
Nimrat, S. (2000). Biodegradation of methyl parathion, p-nitrophenol and p-aminophenol under anoxic conditions (Ph.D. in Environmental Sciences), Department of Enviromental Science, Retgers, TheState University of New Jersey, New Brunswich, USA.
Stickland, J.D.H. & Parson, T.R. (1972). A practical handbook of seawater analysis (2nd ed.). Ottawa: Fisheries Research Board of Canada Bulletin.
Stabili, L., Acquaviva, M. & Cavallo, R.A. (2005). Mytilis alloprovincialis filter feeding on the bacterial community in Mediterranean area (Northern Ionian Sea, Italy). Water Research, 36, 469 - 477.
Trabue, S.L., Ogram, A.V. & Ou, L.T. (2001). Dynamics of carbofuran-degrading microbial communities in soil during three successive annual applications of carbofuran. Soil Biology & Biochemistry, 33, 75 - 81.
Van Schie, P.M. & Young, L.Y. (1998). Isolation and characterization of phenol-degrading denitrifying bacteria. Applied and Environmental Microbiology, 64 (7), 2432 - 243.
Zhang, X. & Wiegel, J. (1994). Reversible conversion of 4-hydroxybenzoate and phenol by Clostridium hydroxybenzoicum. Applied and Environmental Microbiology, 60 (11), 4128 - 4185.
