Biodegradation of Phenolby Microorganisms in Sediment-Slurry under Aerobic Denitrification
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Abstract
In this study, biodegradation of phenol by microorganisms in rice-paddy sediment-slurry which consisted of nitrate (0.031 mM) and nitrite (0.028 mM) was investigated under aerobic denitrifying conditions. In active culture, 0.1 mM phenol which was applied in the first and the second time was biodegraded within 3 and 2 days, respectively. Then 0.3 mM phenol was reapplied into all treatments, the chemical was biodegraded for more than 5 days of the experiment. The detected metabolite of phenol biodegradation was p-hydroxybenzoate which was monitored at day 3, 6 and 9 with the concentration less than 0.01 mM. Furthermore, these microorganisms in sediment-slurry were able to transform nitrate to nitrite.
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