Physico-chemical Properties and Antioxidant Activity of Trisarn Drug Coordinates

Abstract

Trisarn drug coordinates consists of three herbs; Plumbago indica L., Piper wallichi (Miq.) Hand. - Mazz., Piper sarmentosum Roxb. This formula is a traditional remedy with a spicy essence that promotes body warming, blood circulation, and blood dispersion. The purpose of this study was to investigate the physical and chemical properties of the Trisarn drug coordinates in accordance with the methods described in the Thai Herbal Pharmacopoeia (THP), determination of substances by HPLC technique, and antioxidant activities by DPPH, FRAP assays and determination of total phenolic content by Folin-Ciocalteau. The result indicated that the water extract and ethanol extract content values were at least 10.98 and 9.13 percent by weight, respectively. The maximum values for total ash content, acid insoluble ash, and loss on drying were 10.23 w/w, 2.15 w/w, and 7.73 w/w, respectively. The average quantitative study of the Trisarn drug coordinates' active components for plumbagin and piperine reveals 0.784% ± 0.036 and 1.202% ± 0.043 respectively. The DPPH assay for antioxidant activity revealed that Trisarn drug coordinates showed antioxidant activity with an IC₅₀ of 37.99±0.02 µg/mL. The FRAP test for antioxidant activity revealed that the Trisarn drug coordinates contained 0.27±0.15 mg Trolox/g crude extract. Total phenolic content of Trisarn drug coordinates was 38.14±5.30 mg GAE/g extract. This investigation can be used to verify the Trisarn drug coordinates' physical and chemical qualities. This study is recommended for quality control and data acquisition in preparation for the future development of Trisarn drug coordinates as pharmaceuticals or health products.