Antioxidant, Antibacterial and Anti-biofilm Activities of Three Chinese Herbs against Methicillin-resistant Staphylococcus aureus

Authors

  • Worrapannee Powtongsook Department of Industrial Microbiology, Faculty of Science and Technology, Huachiew Chalermprakiet University
  • Watcharin Rangsipanuratn Faculty of Medical Technology, Huachiew Chalermprakiet University
  • Weerachai Tubchan Department of Industrial Microbiology, Faculty of Science and Technology, Huachiew Chalermprakiet University
  • Parinya Semuang Department of Industrial Microbiology, Faculty of Science and Technology, Huachiew Chalermprakiet University
  • Soramon Sutin Department of Physical Science, Faculty of Science and Technology, Huachiew Chalermprakiet University
  • Saowaluck Meesin Faculty of Chinese Medicine, Huachiew Chalermprakiet University

Keywords:

Huang Pai, Da Huang, Ou Mei, antioxidant, antibacterial, anti-biofilm

Abstract

This study evaluated the efficacy of three Chinese herbs against methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible Staphylococcus aureus (MSSA). The antioxidant, antibacterial and anti-biofilm formation of ethanol extract of three Chinese herbs including Huang Pai (Phellodendron chinensis Schneid), Da Huang (Rheum palmatum Linn.), and Ou Mei (Prunus mume (Sieb) Sieb. Et Zucc.) were evaluated. Results from antioxidant activity using DPPH radical scavenging assay showed that Da Huang had the best antioxidant with an IC50 (50% Inhibitory Concentration) value similar to the standard BHT (Butylated hydroxyl toluene). Results from the agar well diffusion and colorimetric broth microdilution tests revealed that crude extracts of Huang Pai and Da Huang had better activities than Ou Mei. The antimicrobial activity of Huang Pai against MSSA was higher than Da Huang as seen by the MIC (Minimum Inhibitory Concentration) value of 0.391 and 1.562 mg/ml, respectively. The anti-MRSA activity of both herbs’ extracts had a  similar MIC value of 1.562 mg/ml. Huang Pai showed the most potent anti-biofilm effect against MSSA and MRSA with 80% inhibition of biofilm formation at 0.0244 and 0.0488 mg/ml, respectively. Results from this study illustrated that Huang Pai and Da Huang have the potential for further development into natural products treating of Staphylococcus aureus infection.

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Published

2023-05-27

How to Cite

1.
Powtongsook W, Rangsipanuratn W, Tubchan W, Semuang P, Sutin S, Meesin S. Antioxidant, Antibacterial and Anti-biofilm Activities of Three Chinese Herbs against Methicillin-resistant Staphylococcus aureus. HCUJOURNAL [Internet]. 2023 May 27 [cited 2024 May 11];27(1):78-90. Available from: https://he01.tci-thaijo.org/index.php/HCUJOURNAL/article/view/255131

Issue

Vol. 27 No. 1 (2023): January - June 2023

Section

Research article

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