Anti-inflammatory and anti-oxidative activities of Stephania pierrei extracts

Authors

  • Sirilak Kamonwannasit
  • Bung-on Prajanban
  • Agarat Kamcharoen Division of Agro-Industrial Product Development, Faculty of Agricultural Technology, Burapha University, Sa Kaeo 27160
  • Parkarang Srimee

Keywords:

Stephania pierrei Diels, sodium starch glycolate, Anti-inflammatory activity, Cytotoxicity, Pro-inflammatory mediators

Abstract

A tuber of Stephania pierrei Diels (Menispermaceae) has been used as a traditional medicine for edema, migraine, and heart related to inflammation. The objective of this study was to determine the anti-oxidative and anti-inflammatory activities of S. pierrei tuber extracts including hot water extract (SH) and ethanolic extract (SE). Hydroxyl radical and superoxide radical scavenging activities were used to analyze their anti-oxidative activities Reverse Transcriptase-polymerase chain reaction (RT-PCR) was used to investigate the gene expression of pro-inflammatory mediators including IL-1β, COX-2, TNF-α, IL-6, and iNOS compared with a housekeeping gene, β-actin.  In addition, the cytotoxicity of extracts against RAW264.7 macrophages was also determined by using an MTT assay..  The results showed that both SE and SH showed high anti-oxidative activity. The SE showed slightly stronger superoxide radical scavenging activity than SH.  For cytotoxic effect, SE showed higher cytotoxicity than SH with IC50 of 96.14±1.79 μg/ml and 191.80±6.08 μg/ml, respectively.  Both extracts as well as the standard compounds, significantly suppressed the gene expression of all pro-inflammatory mediators.  The SE effectively suppressed COX-2 gene expression to the same degree as the SH (IC50 of 50.85±2.15 μg/ml and 56.54±0.97 μg/ml, respectively).   Moreover, the SE showed a stronger inhibitory effect than the SH on the gene expression, especially IL-1β, IL-6 and TNF-α genes.  These results indicated that S. pierrei tuber extracts might decrease inflammation via suppression of pro-inflammatory mediator gene expression and their anti-oxidative activity. Therefore, S. pierrei tubers can be a good candidate for the development of anti-inflammatory health products.

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Published

2022-06-30

How to Cite

1.
Kamonwannasit S, Prajanban B- on, Kamcharoen A, Srimee P. Anti-inflammatory and anti-oxidative activities of Stephania pierrei extracts. HCUJOURNAL [Internet]. 2022 Jun. 30 [cited 2023 Feb. 7];26(1):25-34. Available from: https://he01.tci-thaijo.org/index.php/HCUJOURNAL/article/view/252970

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Research article