Isolation of Lactic Acid Bacteria from Healthy Thai Volunteers Using Throat Swab Test
Keywords:
Isolation, Lactic acid bacteria, Throat, ThaiAbstract
Lactic acid bacteria (LAB) are commonly used as probiotic in human. It is necessary to isolate the LAB in the first step before using the bacteria in various aspects. The objective of this study was to isolate and collect LAB from healthy Thai volunteers by using throat swab test. The isolation of LAB from healthy Thai volunteers was done in the area of Bansomdejchaopraya Rajabhat University and the community surrounding Bansomdejchaopraya Rajabhat University. The isolated LAB were determined for colony morphology, Gram staining, and pH value in the cultured medium.
The results showed that the volunteers could be classified into 3 groups, Thai volunteers in the area of Bansomdejchaopraya Rajabhat University, Thai volunteers in the area of Thai Buddhist community surrounding Bansomdejchaopraya Rajabhat University, and Thai volunteers in the area of Thai Islamic community surrounding BansomdejchaoprayaRajabhat University. The total number of volunteers in 3 groups was 232. The total number of isolated LAB was 188. The colony morphology of these isolates could be classified into 3 types which were small, medium, and large size with the color of opaque-white and smooth convex edge. All LAB were Gram positive. The study of pH value in the cultured medium demonstrated that pH ranging from 3.6-6.2. The analysis of variance of numbers of isolated LAB between volunteer groups showed no statistical significance between groups. In conclusion,throat swab test could use for isolation and collection of LAB Thai strains from healthy Thai volunteers.
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