Detection of ctxA gene of Cholera from human and environment for surveillance at Phop Phra Distric, Tak province.
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Abstract
Vibrio cholerae carrying the ctxAB gene can produce cholera toxin and be the genome of a filamentous
bacteriophage (CTXø). Phop Phap district of TAK province was occasional outbreaks of cholera. A probably
human and some environment may be an inter-epidemic reservoir of V. cholerae 01. This study was surveillance
of ctxA gene of Vibrio cholerae on February 2009 to February 2012 by detection of rectal swab, total samples
3,509 samples, were found LAMP ctxA positive 191 samples 5.4% contain with samples from normal human
2.9%, diarrhoeal patients of Phop Phap hospital 3.8% and contact case of cholera patients 13.3%, while the
culture method found Vibrio cholerae 01, El Tor, Inaba 79 sample 2.3% (normal human 0%, diarrhoeal patients 1.7% and contact case 8.9%) More of this on September 2010 to August 2011 we collected environment sample
600 samples LAMP ctxA positive 14 samples (2.3%) from canal water 10 samples and algae 4 samples. The
result show found ctxA of Vibrio cholerae in normal human, diarrhoeal patients, contact case, canal water and
algae in the endemic area. Then we can believe that were have some hidden of the low number of Vibrio cholerae
01 in human and environment or on state of viable but non-culturable (VNC or VBNC). This experiment can be
a useful data for control and prevention of cholera outbreak in risk group or in other area.
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ข้อลิขสิทธิ์วารสาร
บทความหรือข้อคิดเห็นใดๆ ที่ปรากฏในวารสารวิชาการป้องกันควบคุมโรค สคร. 2 พิษณุโลก เป็นวรรณกรรมของผู้เขียน กองบรรณาธิการวิชาการ และ สำนักงานป้องกันควบคุมโรคที่ 2 จังหวัดพิษณุโลกไม่จำเป็นต้องเห็นพ้องด้วยทั้งหมดหรือร่วมรับผืิดชอบใดๆ
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