Antibiotic susceptibility patterns and detection of extended-spectrum β-lactamase producing Klebsiella pneumoniae isolated from patients in Roi Et hospital, Thailand

Abstract

Extended-spectrum β-lactamase producing Klebsiella pneumoniae strains (ESBLKPs) and multidrug resistant Klebsiella pneumonia (MDRKP) is frequently found in Thailand. The infected patients are difficult to treat and may lead to treatment failure. The aim of this study was to investigate the antibiotic susceptibility patterns of ESBLKPs from the clinical specimens of patients at Roi Et Hospital. The study was conducted between January1, 2014 to October 31, 2015.  The clinical specimens were examined including urine, sputum, pus, body fluids and blood. The antimicrobial susceptibility testing was performed by disk diffusion, and β-lactamase enzymes produced were tested by combined disk diffusion methods and using Klebsiella pneumoniae ATCC 700603 to be a reference strain. All laboratory processes were performed according to guideline of CLSI January, 2010. Descriptive statistics (mean, standard derivation, percent) were used for data analysis. From 125 patients, most of them were female 50.4%, mean age 62.2 years (+SD =15.6), more than 7 days admitted 52.5%. Clinical specimens were sputum 71.2%. Of 90 subjects were diagnosed with ESBLKPs for 98.0%, 98.0%, 98.0%, 97.0%, 97.0%, 72.0% and 81.0% susceptible to amikacin, imipenem, meropenem, ertapenem, doripenam, gentamicin andtigecycline respectively. Also, 35 subjects were identified as ESBL+ MDRKPs and 98.0%, 97.0%, 96.0%, 96.0%, 86.0%, 68.0% respectively which were susceptible to meropenem, doripenem, impipenem, ertapenem, amikacin and tigecycline respectively. In conclusion, this study revealed that ESBLKPs and ESBL+ MDRKPs were high susceptible to carbapenem, amikacin and tigecycline.