Comparative evaluation of antinuclear antibody testing by immunoperoxidase staining and immunofluorescence assay

Abstract

This study aimed to evaluate the method to detect antinuclear antibodies (ANA) by immunoperoxidase staining (IP) comparing with indirect immunofluorescence assays (IFA). A total 164 patient sera composed of 64 positive cases and 100 negative cases by IFA. IP showed 98.44 % sensitivity, 99.00 % specificity and 98.78 % accuracy comparing with IFA. The antibodies titer had high correlation between both methods (P < 0.001, gamma = 0.995). In addition, the staining patterns were also not different (P = 1.000). Therefore, IP had capacity to replace IFA for ANA detection.