Comparative evaluation of antinuclear antibody testing by immunoperoxidase staining and immunofluorescence assay
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Abstract
This study aimed to evaluate the method to detect antinuclear antibodies (ANA) by immunoperoxidase staining (IP) comparing with indirect immunofluorescence assays (IFA). A total 164 patient sera composed of 64 positive cases and 100 negative cases by IFA. IP showed 98.44 % sensitivity, 99.00 % specificity and 98.78 % accuracy comparing with IFA. The antibodies titer had high correlation between both methods (P < 0.001, gamma = 0.995). In addition, the staining patterns were also not different (P = 1.000). Therefore, IP had capacity to replace IFA for ANA detection.
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