Classification of lipooligosaccharide biosynthesis loci and heat-stable serotypes of Campylobacter jejuni isolates from diarrheal children less than 5 years of age in Thammasat Chalermprakiet Hospital Thailand

Abstract

A total of 26 C. jejuni isolates from diarrheal children under 5 years in Thammasat Chalermprakiet Hospital were classified into different groups by lipooligosaccharide (LOS) loci using PCR assay and heat-stable (HS) serotypes using passive hemagglutination technique. Based on LOS loci, 23 isolates were classified into 4 classes, B2 (10 isolates), H (10 isolates), B1 (2 isolates) and C (1 isolate).  However, 3 isolates could not be classified by LOS loci. For HS serotypes, 20 isolates were classified as 8 serotypes, whereas 6 isolates were nontypeable. Serotypes HS: 1,44 and HS: 5 were found in LOS class B2. It was found that classification of LOS loci and HS serotypes may provide a prognosis of patients infected with C. jejuni and HS serotypes were probably specific to LOS classes.