cdtB gene-based multiplex PCR for detection of Campylobacter spp. isolated from rectal swab culture of diarrheal children in Bamrasnaradura Infectious Diseases Institute

Abstract

Multiplex PCR technique was used for detecting the cdtB gene of Campylobacter  spp. isolated from rectal swabs of diarrheal children at Bamrasnaradura Infectious  Diseases Institute. Of the 691 samples analyzed by the multiplex PCR, 28 (4.1 %) were positive for Campylobacter spp.; 24 samples (3.5 %) for Campylobacter jejuni and 4 samples (0.6 %) for Campylobacter coli. The conventional culture method could detect 15 isolates (2.2 %) of C. jejuni   and 1 isolate (0.1 %) of  C. coli  from all rectal swab samples. These data showed that the cdtB gene-based multiplex PCR technique provided better result for detection and identification of Campylobacter spp. than that of the conventional culture method.