Selection of standard platelets for platelet antibody detection

Abstract

Platelet alloantibodies can cause neonatal alloimmune thrombocytopenia (NATP),

post-transfusion purpura (PTP) and platelet transfusion refractoriness. Detection of these antibodies requires the standard platelets with known antigens. In this study, human platelet antigens (HPA) -1 to -6 and HPA-15 alleles were typed in 126 group O single donor platelets (SDP) using PCR with sequence specific primers (PCR-SSP). HPA-1a, -2a, -4a, -5a and -6a were present in all samples. HPA-1b, -2b, -5b, and -6b were rare and HPA-4b was not found. The gene frequencies of HPA-3a, -3b, -15a and -15b were 52.4%, 47.6%, 59.1% and 40.9 %, respectively. The gene frequencies of HPA in this study were similar to those of previous studies in Thais and Asians population. For standard platelet selection, platelets from 3 donors were selected for platelet antibody screening test and 9 donors were selected for platelet antibody identification. This set of standard platelets can identify anti-HPA-1b, -2b, -3a, -3b, -5b, -6b, -15a and -15b. Moreover, the distribution of HPA genotypes in this study provides basic information on donor which will be useful for further searching of HPA- matched platelets for patients.