Antimicrobial activity of extracts of Thai plant to Burkholderia pseudomallei

Abstract

Recent reports of a reduced susceptibility of Burkholderia pseudomallei (B. pseudomallei), the causative agent of melioidosis, to antibiotics, especially ceftazidime, have indicated a need for further research into the effective antimicrobial activity of plants. The aim of this study was to test the antimicrobial activity of 6 plant extracts, Barringtonia acutangula (L.) Gaertn, Cleome gynandra Linn., Luffa acutangula (Linn.) Roxb., Limnophila geoffrayi Bonati, Centella asiatica (L.) Urban, and Piper sarmentosum Roxb. to B. pseudomallei H1038, A2, G207, and from clinical specimen. Crude extracts were tested for their antimicrobial activity by the standard disc diffusion assay and broth dilution method. Methanol, ethyl acetate, and water were used as solvent for extraction. The methanolic extract of Barringtonia acutangula (L.) Gaertn showed the best antimicrobial results against 4 strains of B. pseudomallei with minimum inhibitory concentration (MIC) of 4 mg/ml. Other plant extracts did not have any antimicrobial effects on this organism.