Detection of mutagenicity of biocompound from Bacillus spp. by the Salmonella mutagenicity test

Abstract

The protein concentration of partially purified biocompounds (PPBs) prepared from culture broth of Brevibacillus  laterosporus  SA14, SA15, Bacillus pumilus BA16/2  and Bacillus megaterium BA6 were 1.746, 1.188, 0.355  and 0.242  mg/ml, respectively.  It was found that only PPBs prepared from culture broth of Brev. laterosporus SA14 and SA15 could inhibit the growth of Staphylococcus aureus, Salmonella typhimurium TA98 and TA100. However, PPBs prepared from culture broth of SA14 and SA15 at the titer of 1:128 did not inhibit the growth of TA98 and TA100. The PPBs obtained from all tested Bacillus species were used to determine the mutagenic properties using Ames test. It was found that all PPBs did not show mutagenic properties by this Ames’ test at the mutagenicity index (MI) > 4. However, further tests using other systems should also be carried out to evaluate its useful safety.