Interpretation of hemoglobin analysis for diagnosis of common thalassemia in Thailand

Abstract

At present hemoglobin (Hb) analysis is mainly performed using automated hemoglobin analyzer based on either

high pressure liquid chromatography (HPLC), low pressure liquid chromatography (LPLC) or capillary zone

electrophoresis (CZE). These automated Hb analyzers separate each Hb and quantify its amount by built

in spectrophotometric determination. Hb chromatogram or electrophoregram as well as the amount of each Hb

are shown and could be printed. Interpretation of the result of Hb analysis is usually made by investigator who

performs the test. Accurate interpretation and diagnosis require basic knowledge on both the principle of

the machine used and the heterogeneity of thalassemia which is the most common inherited genetic disorder

in Thailand. Using subject information including age, clinically signs and symptoms, history of blood transfusion

and the result of initial screening test in this interpretation and diagnosis should help in providing more accurate

interpretation and diagnosis of common forms of thalassemia.