Lateral Flow Assay for NGAL Detection Using CysteamineModified Gold Nanoparticles

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Published: Mar 13, 2025
Keywords:
NGAL detection Lateral flow assay Cysteamine-modified gold nanoparticles Kidney disease Point-of-care testing

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Paweena Tunakhun
Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen, Thailand. Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen, Thailand.
Jullasak Daduang
Demonstration School of Khon Kaen University, Secondary Level (Mo Din Daeng), Khon Kaen University, Khon Kaen 40002, Thailand.
Ativit Chueaponthong
Demonstration School of Khon Kaen University, Secondary Level (Mo Din Daeng), Khon Kaen University, Khon Kaen, Thailand.
Tanaton Taveepok
Demonstration School of Khon Kaen University, Secondary Level (Mo Din Daeng), Khon Kaen University, Khon Kaen, Thailand.
Panadda Wongwichittanon
Demonstration School of Khon Kaen University, Secondary Level (Mo Din Daeng), Khon Kaen University, Khon Kaen, Thailand.
Nicha Charoensri
Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Oranee Srichaiyapol
Center for Innovation and Standard for Medical Technology and Physical Therapy (CISMaP), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Patcharaporn Tippayawat
Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Patcharee Boonsiri
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Sirirat Anutrakulchai
Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Kiattawee Choowongkomon
Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Ratree Tavichakorntrakool
Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Pornsuda Maraming
Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Sawinee Ngernpimai
Center for Innovation and Standard for Medical Technology and Physical Therapy (CISMaP), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

Abstract

Neutrophil gelatinase-associated lipocalin (NGAL) is a promising biomarker of chronic kidney disease (CKD). A rapid and cost-effective method is required for CKD detection. This study aimed to develop and evaluate the performance characteristics of an antibody (Ab) and a method for NGAL detection in clinical samples and assess its potential as a point-ofcare test. The developed assay was highly specific for NGAL and showed no cross-reactivity with other endogenous substances. The developed lateral flow immunoassay based on cysteamine-modified gold nanoparticles exhibited a sensitivity of 12.5 ng/mL. The results showed that the dual monoclonal Ab-based sandwich is ideal for screening patients with CKD and has the potential to provide rapid and cost-effective detection of NGAL as a point-of-care testing system.

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How to Cite
1.
Tunakhun P, Daduang J, Chueaponthong A, Taveepok T, Wongwichittanon P, Charoensri N, Srichaiyapol O, Tippayawat P, Boonsiri P, Anutrakulchai S, Choowongkomon K, Tavichakorntrakool R, Maraming P, Ngernpimai S. Lateral Flow Assay for NGAL Detection Using CysteamineModified Gold Nanoparticles. Arch AHS [internet]. 2025 Mar. 13 [cited 2025 Mar. 31];37(1):37-4. available from: https://he01.tci-thaijo.org/index.php/ams/article/view/273646
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Vol. 37 No. 1 (2025): January - April
Section
Original article
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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