Detection of KRAS mutation at codon-12 and codon-13 associate with poor prognosis in colorectal cancer patients

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Namphon Klaewkla
Sarawut Chantra
Chanwit Maneenin
Parichart Pinyosri
Surasak Wanram

Abstract

Colorectal cancer (CRC) associated with KRAS mutations relevance to clinical outcome of progression especially for codon mutation remain unclear, clinically. Thus this study aimed to detect KRAS mutations on specific codon-12 and codon-13. DNA extraction was performed from 40 formalin-fixed paraffin-embedded (FFPE) tissues. The KRAS codon-12 and codon-13 mutations were detected by quantitative multiplex real-time PCR consisting of seven primers of G12A, G12D, G12R, G12C, G12S, G12V, and G13D, specifically. Our results showed mutation of codon-12 at 37.5% (15/40 cases), especially for 86.7% (13/15 cases) were found G12D, G12V and G12R mutation for 40%, 40% and 6.67%, respectively. On the other hand, the codon-13 mutation found only G13D at 20% (3/15 cases). The present findings showed a statistically significant difference between KRAS mutations with lymph node metastases (p-value = 0.004), and mucosal lymphoma with other tumor types (p-value = 0.016). We noticed that KRAS mutations are associated with codon-12 and codon-13 and relevanced to prognostic outcomes of CRC patients on lymph node metastases as well as histological types. However, increased samples, novel prognostic biomarkers and more specific clinical information could be further explored to the clinical molecular biomarkers for monitoring and treatments.

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1.
Klaewkla N, Chantra S, Maneenin C, Pinyosri P, Wanram S. Detection of KRAS mutation at codon-12 and codon-13 associate with poor prognosis in colorectal cancer patients. Arch AHS [Internet]. 2023 Jul. 17 [cited 2024 May 28];35(2):19-24. Available from: https://he01.tci-thaijo.org/index.php/ams/article/view/261693
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Original article

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