Nuclear proteins of hela cells: potential autoantigenic substrate for antinuclear antibodies screening

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Published: Jul 17, 2023
Keywords:
Antinuclear antibody Autoimmune disease Hela cell Nuclear protein Rapid test

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Thiha Thway
Biomedical Sciences Program, Graduates School of Khon Kaen University, Khon Kaen, Thailand. The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Sucheewa Wongwai
Department of Clinical Immunology and Transfusion Sciences, School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Prinya Prasongdee
Clinical Laboratory section, Srinagarind hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Chanvit Leelayuwat
The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand. Department of Clinical Immunology and Transfusion Sciences, School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Amonrat Jumnainsong
The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand. Department of Clinical Immunology and Transfusion Sciences, School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

Abstract

Antinuclear antibodies (ANA) are important in diagnosis and follow-up of patients with autoimmune conditions. The current increase in ANA requests is driven by broadening the use of ANA from a test for lupus to a test for diverse autoimmune diseases, but the standard method is protracted, cumbersome and prone to error. Therefore, simple and reliable testing are needed and autoantigen substrates are required for the development to capture the autoantibodies. This study evaluates the nuclear proteins of Hela cells (NP-HL) as screening marker for systemic autoimmune diseases. Reactivity of 38 ANA positive and 10 negative sera, against NP-HL was determined by western blotting. We demonstrated that NP-HL reacts with 37 ANA positive sera (97%), and without showing any reaction with negative ones. NP-HL was shown to have a diagnostic value as a screening marker for ANA and, therefore, is a suitable alternative substrate for a new antibody test. This research implies that the NP-HL provides a potential to be used as autoantigen substrate in the rapid testing to define ANA for screening of autoimmune diseases.

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1.
Thway T, Wongwai S, Prasongdee P, Leelayuwat C, Jumnainsong A. Nuclear proteins of hela cells: potential autoantigenic substrate for antinuclear antibodies screening. Arch AHS [Internet]. 2023 Jul. 17 [cited 2024 Dec. 19];35(2):12-8. Available from: https://he01.tci-thaijo.org/index.php/ams/article/view/261650
Issue
Vol. 35 No. 2 (2023): May - August
Section
Original article
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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