The potential of rapid molecular RT-PCR for SARS-CoV-2 detection: implementation of the Cobas Liat system with urgent patients during a high-incidence season in Thailand

Main Article Content

Pongwut Suwannarat
Rawiporn Tiyasirichokchai
Pornparn Rojanasang
Wittaya Jomoui

Abstract

There had been a high incidence of COVID-19 in Thailand between May 2021 and February 2022. Urgent cases in the emergency department (ED) or labor room (LR) should be immediately tested. We aim to implement a rapid molecular point-of-care tests (POCTs) using Cobas Liat for SARS-CoV-2 identification. A total of 32 random leftover-samples represented 100% overall concordance among the 2 POCTs and standard RT-PCR assays. A total of 3,188 nasopharyngeal swabs from an ED and LR were investigated with Cobas Liat system. The incidence of SARS-CoV-2 infection was observed to be 7.53% (164/2,179) in ED and 2.87% (29/1,009) in LR. Furthermore, symptomatic cases were noted in 69.51% (114/164) of ED patients, whereas only one case was recorded from the LR. Finally, this potential strategy could reduce turnaround time and the risk of infection in medical staff during the management of patients or medical procedures in the ED and LR.

Article Details

How to Cite
1.
Suwannarat P, Tiyasirichokchai R, Rojanasang P, Jomoui W. The potential of rapid molecular RT-PCR for SARS-CoV-2 detection: implementation of the Cobas Liat system with urgent patients during a high-incidence season in Thailand. Arch AHS [Internet]. 2023 Jul. 17 [cited 2024 May 28];35(2):1-11. Available from: https://he01.tci-thaijo.org/index.php/ams/article/view/261534
Section
Original article

References

Tsang HF, Chan LWC, Cho WCS, Yu ACS, Yim AKY, Chan AKC, et al. An update on COVID-19 pandemic: the epidemiology, pathogenesis, prevention and treatment strategies. Expert Rev Anti Infect Ther 2021; 19(7): 877-88.

Hoffmann M, Kleine-Weber H, Schroeder S, Krüger N, Herrler T, Erichsen S, et al. SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell 2020; 181(2): 271-80.

Chan JF, Kok KH, Zhu Z, Chu H, To KK, Yuan S, et al. Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan. Emerg Microbes Infect 2020; 9(1): 221-36.

Rothan HA, Byrareddy SN. The epidemiology and pathogenesis of coronavirus disease (COVID-19) outbreak. J Autoimmun 2020; 109: 102433.

van Doremalen N, Bushmaker T, Morris DH, Holbrook MG, Gamble A, Williamson BN, et al. Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. N Engl J Med 2020; 382(16): 1564-7.

Lauer SA, Grantz KH, Bi Q, Jones FK, Zheng Q, Meredith HR, et al. The incubation period of coronavirus disease 2019 (COVID-19) from publicly reported confirmed cases: estimation and application. Ann Intern Med 2020; 172(9): 577-82.

Prasertbun R, Mori H, Mahittikorn A, Siri S, Naito T. Pneumonia, influenza, and dengue cases decreased after the COVID-19 pandemic in Thailand. Trop Med Health 2022; 50(1): 27.

Hsu CH, Chiu CL, Lin YT, Yu AY, Kang YT, Cherng M, et al. Triage admission protocol with a centralized quarantine unit for patients after acute care surgery during the COVID-19 pandemic: A tertiary center experience in Taiwan. PLoS One 2019; 17(3): e0263688.

Gdoura M, Abouda I, Mrad M, Ben Dhifallah I, Belaiba Z, Fares W, et al. SARS-CoV2 RT-PCR assays: In vitro comparison of 4 WHO approved protocols on clinical specimens and its implications for real laboratory practice through variant emergence. Virol J 2022; 19(1): 54.

Porte L, Legarraga P, Vollrath V, Aguilera X, Munita JM, Araos R, et al. Evaluation of a novel antigen-based rapid detection test for the diagnosis of SARS-CoV-2 in respiratory samples. Int J Infect Dis 2020; 99: 328-33.

Mak GC, Cheng PK, Lau SS, Wong KK, Lau CS, Lam ET, et al. Evaluation of rapid antigen test for detection of SARS-CoV-2 virus. J Clin Virol 2020; 129: 104500.

Chaimayo C, Kaewnaphan B, Tanlieng N, Athipanyasilp N, Sirijatuphat R, Chayakulkeeree M, et al. Rapid SARS-CoV-2 antigen detection assay in comparison with real-time RT-PCR assay for laboratory diagnosis of COVID-19 in Thailand. Virol J 2020; 17(1): 177.

Er TK, Chou YC, Chen SY, Huang JW. Rapid Cobas Liat SARS-CoV-2 assay in comparison with the laboratory-developed real-time RT-PCR Test. Clin Lab 2021; 67(11): e210316.

Blackall D, Moreno R, Jin J, Plotinsky R, Dworkin R, Oethinger M. Performance characteristics of the roche diagnostics cobas Liat PCR system as a COVID-19 screening tool for hospital admissions in a regional health care delivery system. J Clin Microbiol 2020; 59(10): e0127821.

Hansen G, Marino J, Wang ZX, Beavis KG, Rodrigo J, Labog K, et al. clinical performance of the point-of-care cobas liat for detection of SARS-CoV-2 in 20 minutes: a multicenter study. J Clin Microbiol 2021; 59(2): e02811-20.

Granato PA, Kimball SR, Alkins BR, Cross DC, Unz MM. Comparative evaluation of the Thermo fisher TaqPath™ COVID-19 combo kit with the Cepheid Xpert® Xpress SARS-CoV-2 assay for detecting SARS-CoV-2 in nasopharyngeal specimens. BMC Infect Dis 2021; 21(1): 623.

Graham M, Williams E, Isles N, Buadromo E, Toatu T, Druce J, et al. Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay. Diagn Microbiol Infect Dis 2021; 99(2): 115238.

Hin FT, Wai L, Lawrence C, William C, Sze C. Performance comparison of the Cobas® Liat® and Cepheid® GeneXpert® systems on SARS-CoV-2 detection in nasopharyngeal swab and posterior oropharyngeal saliva. Expert Rev Mol Diagn 2021; 21(15): 515-8.

Kim H, Hegde S, LaFiura C, Raghavan M, Sun N, Cheng S, et al. Access to personal protective equipment in exposed healthcare workers and COVID-19 illness, severity, symptoms and duration: a population-based case-control study in six countries. BMJ Glob Health 2021; 6(1): e004611.

Park SH. Personal protective equipment for healthcare workers during the COVID-19 pandemic. Infect Chemother 2020; 52(2): 165-82.

Raju S, Anderson NW, Robinson E, Squires C, Wallace MA, Zhang R, et al. Comparison of 6 SARS-CoV-2 molecular methods and correlation with the cycle threshold distribution in clinical specimens. J Appl Lab Med 2021; 6(6): 1452-62.

Sakano T, Urashima M, Takao H, Takeshita K, Kobashi H, Fujiwara T. Differential kinetics of cycle threshold values during admission by symptoms among patients with mild COVID-19: a prospective cohort study. Int J Environ Res Public Health 2021; 18(15): 8181.