Establishment of in-house telomere length measurement using qPCR

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Published: Dec 23, 2022
Keywords:
Telomere length Chronological age Quantitative polymerase chain reaction (qPCR)

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Buntarawan Turapra
Faculty of Graduate School, Khon Kaen University, Khon Kaen, Thailand.
Jureerut Daduang
School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Molin Wongwattanakul
School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand. Center for Innovation and Standard for Medical Technology and Physical Therapy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Chul-Young Bae
Department of Mediage R&D Center, Corporation Growth Center, Seong-nam, South Korea.
Patcharee Jearanaikoon
School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand. Center for Innovation and Standard for Medical Technology and Physical Therapy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

Abstract

Telomere is a nucleoprotein complex at the ends of chromosomes which can be used as a biomarker for aging and health condition. The gold standard method for telomere length measurement is quite a complication. Recently, quantitative polymerase chain reaction (qPCR) is a widely used method for molecular study with high throughput and cost-effectiveness. This study aimed to develop the in-house qPCR for estimating telomere length in kilobase in healthy samples. The in-house qPCR was established for the telomere gene and 36B4 gene. The analytical performance was verified prior to applying to 190 healthy participants, 139 females and 51 males. The telomere length (kilobase) was calculated and compared to the reference value. The results for telomere length by in-house qPCR method were 7.48 ± 1.78 kb for males and 7.53 ± 1.45 kb for females with the range of 4.66 – 10.69 kb and 4.03 – 11.50 kb, respectively. A total of 190 participants showed moderate correlation with a reference value at R2 = 0.5672. The Bland-Altman analysis from two different methods showed only 5.26% (10 out of 190) were out of ± 25% bias in females. The in-house qPCR was successfully demonstrated for telomere length measurement with an acceptable performance compared to the reference values. However, the validation in more clinical samples should be performed in further study.

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1.
Turapra B, Daduang J, Wongwattanakul M, Bae C-Y, Jearanaikoon P. Establishment of in-house telomere length measurement using qPCR. Arch AHS [Internet]. 2022 Dec. 23 [cited 2024 Dec. 18];34(3):44-52. Available from: https://he01.tci-thaijo.org/index.php/ams/article/view/258097
Issue
Vol. 34 No. 3 (2022): September - December
Section
Original article
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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