Toxicity screening to human peripheral blood mononuclear cells and Wistar rats by Crocodylus siamensisliver extracts
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Abstract
Crocodile organs have been used as ingredients in traditional medicine in Asia for prevention and treatment of diseases. This study, Crocodylus siamensis liver was extracted to evaluate cytotoxicity and immune responses in Wistar rats. The soluble proteins part of C. siamensis’ liver was prepared. In vitro cytotoxicity was assayed in human peripheral blood mononuclear cells (hPBMCs). In vivo chronic toxicity test was performed on Wistar rats with single dose. Body weights, serum biochemical parameters, serum cytokines and the histopathology were observed. The 50% cytotoxic concentration (CC50) of Crude Liver crocodile Extract (CLE) is 28.41 mg/ml. Oral administration showed no toxicity and no adverse effects for chronic period. Serum cytokines did not differ significantly between Th1 and Th2. We found the CC50 of the CLE in hPBMCs. No toxicity was observed in vivo at the study concentration, with the pro-inflammatory cytokines skewed. Since the using of traditional medicine as an alternative therapy is becoming more widely available, this study is an important beginning step to determine the toxicity of bioactive substances which found non-toxic to cells and animal model. For further study, the effectiveness of a biomolecule is compulsorily to identify. To research on their property and develop to supplement products.
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