The genotypic distribution of drug resistant Mycobacterium tuberculosis strains isolated from Northern region of Myanmar
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Abstract
Myanmar is one of both 30 high TB and MDR-TB burden countries worldwide. While most studies have expressed distribution of Mycobacterium tuberculosis genotypes in Lower Myanmar, there has been little research in genetic diversity of M. tuberculosis in Northern region. The objective of this study was to determine the genotypic distribution of drug resistant M. tuberculosis strains isolated from Northern region of Myanmar. Sixty-fve isolates were randomly collected from TB Reference Laboratory of Northern region of Myanmar between August 2016 and December 2017. All isolates were genotyped by using 24-locus Mycobacterial Interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing. The results
showed MIRU-VNTR typing classifed 64 different patterns: 63 isolates had unique MIRU-VNTR profle and 2 isolates were grouped into one cluster. We found that the most prominent strains were Beijing lineages (n = 58, 89.23%) and the other included EAI (n = 2, 3.08%), Delhi/CAS (n = 1, 1.54%), and Unknown strains (n = 4, 6.15%). The overall discriminatory power of all strains showed 0.9995. The allelic diversity of each locus was predictable by HGDI index. Mtub21, Qub2163b, MIRU 26, QUB26 showed HGDI > 0.6 that were recognized as highly discriminatory power. In conclusion, 24-locus MIRU-VNTR offered high discriminatory power within tested isolates. Our fndings showed Beijing genotypes were dominant in Northern region of Myanmar. The analysis of 24-locus MIRU-VNTR typing might be useful for broader understanding of TB outbreaks and epidemiology in this region.
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