Prebiotic capsules containing anthocyanin, inulin and rice bran extracts increased plasma ascorbate of overweight or obese subjects

Main Article Content

Panicha Pongnaratorn
Yupaporn Kanpetta
Ploypailin Aneknan
Juntanee Uriyapongson
Panakaporn Wannanon
Charnchai Panthongviriyakul
Naruemon Leelayuwat

Abstract

Prebiotic foods have an important role in health well-being, especially for treating overweight or obesity by promoting gut microbiota to improve antioxidant activity and fat metabolism. We aimed to investigate the effect of prebiotic capsules containing anthocyanin, inulin and rice bran extract on antioxidant, leptin and insulin resistance, and fat metabolism of overweight or obese subjects. The research was a randomized double-blinded controlled trial. Sixty overweight or obese subjects were randomly allocated into two groups; 1) 350 mg/prebiotic capsule (prebiotic group) or 2) 350 mg/maltodextrin (placebo group), 3 capsules after each meal and before going to bed (4.2 g.day-1) for 30 days. Before and after 30 days of supplementation, anthropometry and body composition were measured. Blood samples were collected to analyze ascorbate, glucose, lipid profles, leptin and insulin concentrations. An expired gas was collected for 5 min to determine substrate utilization. The results showed prebiotic capsules increased plasma ascorbate
concentration (placebo group: before 34.6 ± 3.33 and after 45.2 ± 4.48 μmol/L; treatment group: before 35.7 ± 4.43 and after 62.3 ± 6.47 μmol/L, 95% CI 16.1(4.27-27.9), p-value < 0.05). Comparing within group, hip circumference, lipid profles, and leptin and insulin concentrations were signifcantly decreased with prebiotic supplementation comparing to the baseline data (p-value < 0.05). However, there were no signifcant changes from baseline in fat and carbohydrate oxidation rates, plasma glucose, liver and kidney functions within and between groups (p-value < 0.05). In conclusion, supplementation of prebiotic capsule containing 1.89 g anthocyanin, 1.89 g inulin and 0.42 g rice bran per day for 30 days increased plasma ascorbate concentration in overweight or obesity subjects. Daily supplementation of 4.2 g prebiotics for 30 days did not cause liver and kidney dysfunctions. However, either higher dose or longer duration of the supplementation is warranted to show more variables those are signifcant and to confrm the safety.

Article Details

How to Cite
1.
Pongnaratorn P, Kanpetta Y, Aneknan P, Uriyapongson J, Wannanon P, Panthongviriyakul C, Leelayuwat N. Prebiotic capsules containing anthocyanin, inulin and rice bran extracts increased plasma ascorbate of overweight or obese subjects. Arch AHS [Internet]. 2021 Apr. 23 [cited 2024 Mar. 19];33(1):19-33. Available from: https://he01.tci-thaijo.org/index.php/ams/article/view/242066
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Original article

References

Chooi YC, Ding C, Magkos F. The epidemiology of obesity. Metab Clin Exp 2019; 92: 6-10.

Pan WH, Yeh WT. How to defne obesity? Evidence-based multiple action points for public awareness, screening, and treatment: an extension of Asian-Pacifc recommendations. Asia Pac J Clin Nutr 2008; 17(3): 370-4.

Block G, Mangels AR, Patterson BH, Levander OA, Norkus EP, Taylor PR. Body weight and prior depletion affect plasma ascorbate levels attained on identical vitamin C intake: a controlled-diet study. J Am Coll Nutr 1999; 18(6): 628-37.

Wonisch W, Falk A, Sundl I, Winklhofer-Roob BM, Lindschinger M. Oxidative stress increases continuously with BMI and age with unfavourable profles in males. Aging Male 2012; 15(3): 159-65.

Qiao Y, Sun J, Ding Y, Le G, Shi Y. Alterations of the gut microbiota in high-fat diet mice is strongly linked to oxidative stress. Appl Microbiol Biotechnol 2013; 97(4): 1689-97.

Turnbaugh PJ , Ley RE , Mahowald MA , Magrini V, Mardis ER, Gordon JI. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 2006; 444(7122): 1027-31.

Tucakovic L, Colson N, Santhakumar AB, Kundur AR, Shuttleworth M, Singh I. The effects of anthocyanins on body weight and expression of adipocyte’s hormones: Leptin and adiponectin. J Funct Foods 2018; 45: 173-80.

Anhe FF, Roy D, Pilon G, Dudonne S, Matamoros S, Varin TV, et al. A polyphenol-rich cranberry extract protects from diet-induced obesity, insulin resistance and intestinal infammation in association with increased Akkermansia spp. population in the gut microbiota of mice. Gut 2015; 64(6): 872-83.

Stenholm S, Head J, Aalto V, Kivimaki M, Kawachi I, Zins M, et al. Body mass index as a predictor of healthy and disease-free life expectancy between ages 50 and 75: a multicohort study. Int J Obes (Lond) 2017; 41(5): 769-75.

Delzenne NM, Neyrinck AM, Backhed F, Cani PD. Targeting gut microbiota in obesity: effects of prebiotics and probiotics. Nat Rev Endocrinol 2011; 7(11): 639-46.

Hongu N, Kitts DD, Zawistowski J, Dossett CM, Kopec A, Pope BT, et al. Pigmented rice bran and plant sterol combination reduces serum lipids in overweight and obese adults. J Am Coll Nutr 2014; 33(3): 231-8.

Oki T, Masuda M, Kobayashi M, Nishiba Y, Furuta S, Suda I, et al. Polymeric procyanidins

as radical - scavenging components in red-hulled rice. J Agric Food Chem 2002; 50(26): 7524-9.

Hu C, Zawistowski J, Ling W, Kitts DD. Black rice (Oryza sativaL. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J Agric Food Chem 2003; 51(18): 5271-7.

Lee M, Sorn SR, Park Y, Park HK. Anthocyanin rich-black soybean testa improved visceral fat and plasma lipid profles in overweight/obese Korean adults: a randomized controlled trial. J Med Food 2016; 19(11): 995-1003.

Jamar G, Estadella D, Pisani LP. Contribution of anthocyanin-rich foods in obesity control through gut microbiota interactions. Biofactors 2017; 43(4): 507-16.

Papathanasopoulos A, Camilleri M. Dietary fiber supplements: effects in obesity and metabolic syndrome and relationship to gastrointestinal functions. Gastroenterology 2010; 138(1): 65-72 e1-2.

Rebello CJ, Burton J, Heiman M, Greenway FL. Gastrointestinal microbiome modulator improves glucose tolerance in overweight and obese subjects: A randomized placeboled pilot trial. J Diabetes Complicat 2015; 29(8): 1272-6.

Kulkarni SS, Salehzadeh F, Fritz T, Zierath JR, Krook A, Osler ME. Mitochondrial regulators of fatty acid metabolism reflect metabolic dysfunction in type 2 diabetes mellitus. Metab Clin Exp 2012; 61(2): 175-85.

Daniele G, Xiong J, Solis-Herrera C, Merovci A, Eldor R, Tripathy D, et al. Dapaglifozin Data are expressed as mean ± SE; n=30 in each group (placebo group: 25 females, 5 males and prebiotic group: 26 females, 4 males). CHO; carbohydrate. Diabetes care 2016; 39(11): 2036-41.

Odumosu A, Wilson CW. Hypocholesterolemic effects of vitamin C, clofibrate and diosgenin in male guinea-pigs [proceedings]. Br J Pharmacol 1979; 67(3): 456P-7P.

Ginter E, Zdichynec B, Holzerova O, Ticha E, Kobza R, Koziakova M, et al. Hypocholesterolemic effect of ascorbic acid in maturityonset diabetes mellitus. Int J Vitam Nutr Res Suppl 1978; 48(4): 368-73.

Parnell JA, Reimer RA. Weight loss during oligofructose supplementation is associated with decreased ghrelin and increased peptide YY in overweight and obese adults. Am J Clin Nutr 2009; 89(6): 1751-9.

Zhang H, Li J, Wang K, Du X, Li Q. A simple and sensitive assay for ascorbate using potassium ferricyanide as spectroscopic probe reagent. Anal Biochem 2009; 388(1): 40-6.

Peronnet F, Massicotte D. Table of nonprotein respiratory quotient: an update. Can J Sport Sci 1991; 16:23-9.

Zhou C, Na L, Shan R, Cheng Y, Li Y, Wu X, et al. Dietary vitamin C intake reduces the risk of type 2 diabetes in Chinese adults: HOMA-IR and T-AOC as potential mediators. PLoS One 2016; 11(9): e0163571.

Hassimotto NM, Pinto Mda S, Lajolo FM. Antioxidant status in humans after consumption of blackberry (Rubus fruticosus L.) juices with and without defatted milk. J Agric Food Chem 2008; 56(24): 11727-33.

Bakuradze T, Tausend A, Galan J, Maria Groh IA, Berry D. Antioxidative activity and health benefits of anthocyanin-rich fruit juice in healthy volunteers. Free Radic Res 2019: 1-11.

Ellinger S, Gordon A, Kurten M, Jungfer E, Zimmermann BF, Zur B, et al. Bolus consumption of a specifically designed fruit juice rich in anthocyanins and ascorbic acid did not influence markers of antioxidative defense in healthy humans. J Agric Food Chem 2012; 60(45): 11292-300.

Shang HM, Zhou HZ, Yang JY, Li R, Song H, Wu HX. In vitro and in vivo antioxidant activities of inulin. PloS one 2018; 13(2): e0192273.

Aliasgharzadeh A, Khalili M, Mirtaheri E, Pourghassem Gargari B, Tavakoli F, Abbasalizad Farhangi M, et al. A combination of prebiotic inulin and oligofructose improve some of cardiovascular disease risk factors in women

with type 2 diabetes: a randomized controlled clinical trial. Adv Pharm Bull 2015; 5(4): 507-14.

Hasan M, Damayanthi E, Anwar F. Improvement of antioxidant status on adults obesity after intervention antioxidant drinks. IOP Conf Ser: Earth Env 2018; 196.

Liu C, Sun J, Lu Y, Bo Y. Effects of anthocyanin on serum lipids in dyslipidemia patients: a systematic review and meta-analysis. PloS one 2016; 11(9): e0162089.

Balcazar-Munoz BR, Martinez-Abundis E, Gonzalez-Ortiz M. Effect of oral inulin administration on lipid profile and insulin sensitivity in subjects with obesity and dyslipidemia. Rev med Chile 2003; 131(6): 597-604.

Ooi LG, Liong MT. Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings. Int J Mol Sci 2010; 11(6): 2499-522.

Van der Beek CM, Canfora EE, Kip AM, Gorissen SHM, Olde Damink SWM, van Eijk HM, et al. The prebiotic inulin improves substrate metabolism and promotes short-chain fatty acid production in overweight to obese men. Metabolism Clin and Exp 2018; 87:25-35.

Jackson KG, Taylor GR, Clohessy AM, Williams CM. The effect of the daily intake of inulin on fasting lipid, insulin and glucose concentrations in middle-aged men and women. Br J Nutr 1999; 82(1): 23-30.

Castro-Sánchez FH, Ochoa-Acosta DA, Valenzuela-Rubio NG, Domínguez-Rodríguez M, FierrosValdez JA and Vergara-Jiménez MJ. Inulin effect on weight loss and associated parameters with the development of cardiovascular disease in obese dyslipidemic subjects. Austin J Nutr Metab 2017; 4(1): 1044.

Mahadita WG, Jawi M, Suastika K. Purple sweet potato tuber extract lowers malondialdehyde and improves glycemic control in subjects with type 2 diabetes mellitus. Glob Adv Res J Med Sci 2016; 5(7): 208-13.

Qureshia AA, Sami SA, Khan FA. Effects of stabilized rice bran, its soluble and fiber fractions on blood glucose concentrations and serum lipid parameters in humans with diabetes mellitus Types I and II. J Nutr Biochem 2002; 13(3): 175-87.

Van der Beek CM, Canfora EE, Ellen EB, Cornelis HD, Kaatje L. The prebiotic inulin enhances fat oxidation and improves metabolic parameter in overweight male. Metabolism 2018; 87: 25-35.

Guess ND, Dornhorst A, Oliver N, Bell JD, Thomas EL, Frost GS. A randomized controlled trial: the effect of inulin on weight management and ectopic fat in subjects with prediabetes. Nutr Metab 2015; 12: 36.

Chambers ES, Byrne CS, Morrison DJ, Murphy KG, Preston T, Tedford C, et al. Dietary supplementation with inulin-propionate ester or inulin improves insulin sensitivity in adults with overweight and obesity with distinct effects on the gut microbiota, plasma metabolome and systemic inflammatory responses: a randomised cross-over trial. Gut 2019; 68(8): 1430-8.

Farhangi MA, Javid AZ, Dehghan P. The effect of enriched chicory inulin on liver enzymes, calcium homeostasis and hematological parameters in patients with type 2 diabetes mellitus: a randomized placebo - controlled trial. Prim Care Diabetes 2016; 10(4): 265-71.

Shakib MC, Shreef G, Gamal G. Rice bran oil compared to atorvastatin for treatment of dyslipidemia in patients with type 2 diabetes. Open Access Maced J Med Sci 2014; 2(1): 95-102.

Tashiro H, Kasahara DI, Osgood RS, Brown T, Cardoso A, Cho Y, et al. Sex differences in the impact of dietary fiber on pulmonary responses to ozone. Am J Respir Cell Mol 2020; 62(4): 503-12.