Alteration of natural killer cell subset proportion is associated with risk factors of coronary artery disease in aging

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Published: Aug 27, 2020
Keywords:
Immunosenescence NK cell Coronary artery disease Chronic low-grade inflammation

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Kanuengnit Srisak
Biomedical Sciences Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand.; The Centre of Research and Development of Medical Diagnostic Laboratory, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Wisitsak Phoksawat
The Centre of Research and Development of Medical Diagnostic Laboratory, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.; Graduate School, Khon Kaen University, Khon Kaen, Thailand.
Nantarat Komanasin
Cardiovascular Research Group, Khon Kaen University, Khon Kaen, Thailand.; School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Chanvit Leelayuwat
The Centre of Research and Development of Medical Diagnostic Laboratory, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.; School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Amonrat Jumnainsong
The Centre of Research and Development of Medical Diagnostic Laboratory, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.; School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

Abstract

Currently, the world population is moving to aging society because of decreasing fertility rates and increasing survival in old age. In Thailand, the results of the National Statistics Office showed that the number and proportion of older people increase continuously. Age-related impairment of human immunity involves in many chronic inflammatory diseases such as coronary artery disease (CAD). Thus, age is one of the risk factors for CAD causing the chronic inflammation condition. Natural killer (NK) cell is one of the immune cells that change in aging. Thus, this study aims to investigate the alteration of NK cells and NK cell subsets in aging and their association with the risk factor of CAD. Blood samples from aging (≥ 60 years old) group and who had any risk factors for CAD, were collected. Additionally, blood samples of young group (age < 35 years old) without risk factors associated with CAD were included. Whole blood from these samples were stained with anti-CD3 and anti-CD56 labeled with FITC and PerCP, respectively to determine NK cell subsets by flow cytometry technique. The result showed that the proportion of NK cell subsets (CD56bright/CD56dim) was significantly decreased in aging (p-value < 0.001) and also tended to decrease in aging who had more than or equal to 2 CAD risk factors when compared with aging who had less than 2 CAD risk factors (p-value = 0.051). Thus, this result suggested that alteration of NK cell subsets in aging was associated with the risk factor of CAD and this might be the marker for CAD that needs to be investigated further.

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1.
Srisak K, Phoksawat W, Komanasin N, Leelayuwat C, Jumnainsong A. Alteration of natural killer cell subset proportion is associated with risk factors of coronary artery disease in aging. Arch AHS [Internet]. 2020 Aug. 27 [cited 2024 Jul. 18];32(2):8-16. Available from: https://he01.tci-thaijo.org/index.php/ams/article/view/234260
Issue
Vol. 32 No. 2 (2020): May - August (First issue for 'All in English' under the title 'Arch AHS')
Section
Original article
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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