香附醋制入肝改善非酒精性脂肪肝的网络药理学分析
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摘要
为研究香附醋制后可引药入肝达到增效目的,本研究采用网络药理学方法探析香附醋制入肝改善非酒精性脂肪肝的作用机制。笔者整理近 10 年国内外相关文献,并基于 TCMSP 数据库筛选获得醋香附的有效成分 16 个,相关作用靶点 471 个;利用 GeneCards、OMIM 及 DrugBank 数据库获得非酒精性脂肪肝的靶点基因 1,941 个,其中药物与疾病的共同靶点 104 个;利用 Cytoscape 3.10.0 软件构建 “药物-成分-基因靶点”网络,得到金银花醇、异鼠李素、山奈酚等为香附醋制入肝作用于非酒精性脂肪肝的主要活性成分;将潜在作用靶点导入 String 数据库进行蛋白质相互作用分析,构建 PPI 网络,分析发现 AKT1、JUN、IL6、IL1B 等为关键靶点基因;对作用靶点进行 GO 功能富集分析及 KEGG 通路分析,结果显示醋香附改善非酒精性脂肪肝与多条肝病相关代谢通路相关,包括肿瘤坏死因子 (TNF signaling pathway)、促分裂素原活化蛋白激酶 (MAPK signaling pathway)、磷脂酰肌醇 3-激酶-蛋白激酶 B (PI3K-Akt signaling pathway)信号通路。研究结论显示醋香附可通过金银花醇、异鼠李素、山奈酚等活性成分,作用于 AKT1、JUN、IL6、IL1B 等核心靶点,调控 TNF、MAPK、PI3K-Akt 等肝病相关信号通路,经醋制入肝而改善非酒精性脂肪肝。
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