Identification of Specific Biomarker Genes for Separating Intrahepatic Cholangiocarcinoma Type
Intrahepatic cholangiocarcinoma (ICC) is one of the most lethal liver cancers. ICC is classified into 3 types: mass-forming (MF), periductal infiltrating (PI), and intraductal growth (IG). Among these, the PI and IG types are located in the bile duct, so that histopathologic diagnosis may be quite difficult. This study aims to identify the specific biomarkers for distinguish between PI and IG types. The top five up-regulated genes from PI (C19ORF33, UPK1B, CTHRC1, GREM1, KLK11) and IG type (CFTR, SLC5A1, MUC17, BDKRB2, CCL18) were examined in ICC samples (11 PI and 11 IG samples) using SYBR green-based real-time reverse transcription-PCR (qRT-PCR). Among these genes, the results showed that the CTHRC1 gene of the PI type identified with the highest up-regulation in PI (72.7%) and 27.3% in IG samples (P=0.034), whereas the CFTR gene of the IG type showed the highest up-regulation in 72.7% of IG and 9.1% of PI samples (P=0.001). Hence, the up-regulation of CTHRC1 and CFTR may serve as biomarkers for separating PI from IG types, respectively, and is also a potential diagnostic in ICC. (Thai Cancer J 2020;40:76-85)
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