Plasma MMP-13 Level in Patients with Invasive Ductal Breast Cancer

Authors

  • Nuntana Meesiripan Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University
  • Nonglucksanawan Ritthisunthorn epartment of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University
  • Anong Tepsuwan Biomarker Development Section, Research Division, National Cancer Institute
  • Wassana Tangthai Out Patient Department, National Cancer Institute
  • Thitiluck Swangsri Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University
  • Tipparat Thiangtrongjit Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University
  • Jaree Svedginda Out Patient Department, Hospital for Tropical Diseases, Faculty of Tropical Medicine, Mahidol University
  • Santi Maneewatchararangsri Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University
  • Porntip Chavalitchewinkoon-Petmitr Department of Protozoology, Faculty of Tropical Medicine, Mahidol University
  • Somchai Thanasitthichai Department of Surgery, National Cancer Institute
  • Songsak Petmitr Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University

Keywords:

invasive ductal breast cancer, MMP-13, plasma biomarker

Abstract

Matrix metalloproteinases (MMPs) are a family of zinc-proteolytic endopeptidase enzymes that play an important role in extracellular matrix (ECM) degradation in human cancer progression and may be new targets for cancer therapeutics. This study aimed to detect the plasma level of matrix metalloproteinases 13 (MMP-13) in 108 patients with invasive ductal carcinoma (IDC) and 131 healthy controls with commercial enzyme-linked immunosorbent assay (ELISA) kits. The correlations between these protein levels and the clinical characteristics of the patients were also analyzed to elucidate their role in breast-cancer development. The results showed a significantly lower median concentration of MMP-13 in patients compared with controls (89.58 and 289.15 pg/ml, respectively; P=0.000). Receiver-operator characteristic (ROC) curve analysis, to determine a cutoff value for plasma MMP-13 concentration in the patients, had an AUC of 0.98 (95% CI=0.97-0.99), with a sensitivity of 91.6% and specificity of 91.7% at cutoff point 169 pg/ml. The results underline the difference in plasma level MMP-13 concentration in IDC development, and may serve as a biomarker for patients.

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Published

2017-09-29

Issue

Vol. 37 No. 3 (2017): July – Septemner

Section

Original Articles

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