Antioxidant and Antiproliferative Activities of Carissa carandas Linn. Fruits


  • Suchanuch Ondee Cells and Immunotherapy Section, Research Division, National Cancer Institute


Carissa carandas Linn., antioxidant activity, antiproliferative activity, cancer cell lines


Carissa carandas Linn. (family Apocynaceae), known in Thai as "Nam Daeng", has been recognized among some traditional medicine systems to cure various diseases, such as diarrhea and helminth infections, used as an analgesic, anti-inflammatory, anti-pyretic, and for liver diseases, rheumatism, and cancer. The purpose of this study was to determine the antioxidant and antiproliferative activities of C. carandas juice and methanolic extracts of C. carandas. Fresh ripe fruits of C. carandas were crushed and the juice extract was dried by lyophilizer. The residues of the crushed fruits were then macerated with methanol and the combined extracts evaporated to dryness. The juice and methanolic extracts were used for analysis. The antioxidative potentials of these extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical (OH ) scavenging assays. Gallic acid and mannitol, antioxidant agents were used as positive controls. Antiproliferative activity was also analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay against human cancer cell lines; KB (human epidermoid carcinoma), MCF-7 (human breast adenocarcinoma), SiHa (human cervical carcinoma) and HepG2 (human hepatocellular carcinoma) cell lines, as well as non-tumorigenic cells; Vero (African green monkey kidney) cell line. The results demonstrated that both juice and methanolic extracts of C. carandas fruits exhibited DPPH radical scavenging actions in a dose-dependent manner. Furthermore, the extracts showed hydroxyl radical scavenging activities. However, they showed weak antiproliferative activity toward any of the cells tested. It was concluded that C. carandas fruits possess antioxidative properties and may offer further application as chemopreventive agents


Bhaskar HV, Balakrishnan N. In vitro antioxidant property of laticiferous plant species from Western Ghats Tamilnadu, India. Int J Health Res 2009;2:16370.

Pavithra K, Vadivukkarasi S. Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Science and Human Wellness 2015;4:42-6.

Mukhopadhyay D, Dasgupta P, Roy DS, Palchoudhuri S, Chatterjee I, Ali S, et al. A sensitive In vitro spectrophotometric hydrogen peroxide scavenging assay using 1,10-phenanthroline. Free Rad Antiox 2016;6:124-32.

Anupama N, Madhumitha G. Potential health benefits of Carissa carandas dried fruit methanl extract. J Chem Pharm Res 2015;7:841-7.

Sarma A, Sarmah P, Kashyap D, Dutta S and Mahanta M. Antioxidant activity and nutraceutical property of the fruits of an ethno-medicinal plant: Carissa carandas L. found in Brahmaputra valley agroclimatic condition. J Pharm Sci & Res 2015;7:55-7.

Srivastava PK, Pandey AK. Natural product and derivatives: biological and pharmacological activities. Biochem Cell Arch 2015;15:1-38.

Haidan Y, Qianqian M, Li Y, Guangchun P. The traditional medicine and modern medicine from natural products. Molecules 2016;21:1-18.

Tamrat T, Yesudass DR. Traditional Uses, Pharmacological Action and Phytochemical Analysis of Carissa carandas Linn.: Nat Prod Chem Res 2018; 6:1-20.

Reshu V, Tarun V, Charan S, Geeta S, Jyoti G. Hidden Potential of Natural Herb Carissa Carandas (Karonda). Research in Pharmacy and Health Sciences 2017;3:294-302.

Khatun M, Habib MR, Rabbi MA, Amin R, Islam MF, Nurujjaman M, Karim MR, Rahman MH. Antioxidant, cytotoxic and antineoplastic effects of Carissa carandas Linn. leaves. Exp Toxicol Pathol 2017;69: 469-76.

Yuttana S, Kowit S. In vitro antioxidant, antibacterial, and cytotoxicity activities from karanda (Carissa carandas L.) fruit extracts. Int J Green Pharm 2017;11:S189-93.

Singh A, Uppal GK. A review on Carissa carandasphytochemistry, ethno-pharmacology, and micropropagation as conservation strategy. Asian J Pharm Clin Res 2015;8:26-30.

Arif M, Kamel M, Jawaid T, Khalid M, Saini KS, Kumar A, et al. Carissa carandas Linn. (Karonda): An exotic minor plant fruit with immense value in nutraceutical and pharmaceutical industries. Asian J Biomed Pharm Sci 2016;6:14-9.

Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complement Altern Med 2008;8:1-10.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth 1983;65:55-63.

Mishra CK, Sasmal D, Kumar D. In Vitro Antioxidant activity of chloroform and ethanolic fruit and root extracts of Carissa Carandas Linn. J Innov Pharm Sci 2017;1:41-5.

Dhar G, Akther S, Sultana A, May U, Islam MM, Dhali M, Sikdar D. Effect of extraction solvents on phenolic contents and antioxidant capacities of Artocarpus chaplasha and Carissa carandas fruits from Bangladesh. J Appl Bio & Biotech 2017;5:39-44.

Kumar V, Tarpada P, Sadariya K, Goswami S. Comparative phytochemical and antioxidant activities of methanol and petroleum ether extract of Carissa carandas leaves, fruit and seed. Vivechan Int J Res 2017;8:70-5.

Afizah IN, Ahmad W, Jamshed F, Al-Jasabi. Quantitative biochemical analysis of antioxidant properties of Carissa carandas fruit ethanolic and n-hexane extracts. Middle-East J of Sci Res 2016;24:2418-23.

Khunchalee J, Charoenboon P. Study of free radical scavenging, total phenolic contents and Tyrosinase inhibition activity of crude extract from Carissa carandas Linn. SNRU J Sci Tech 2019;11:26-34.

Pewlong W, Sajjabut S, Eamsiri J, Chookaew S. Evaluation of antioxidant activities, anthocyanins, total phenolic content, vitamin C content and cytotoxicity of Carissa carandas Linn. CMUJ NS Special Issue on Food and Applied Bioscience 2014;13:509-17.

Abdel-Hameed ES, Salih A. Bazaid SA, Shohayeb MM, El-Sayed MM, El-Wakil EA. Phytochemical studies and evaluation of antioxidant, anticancer and antimicrobial properties of Conocarpus erectus L. growing in Taif, Saudi Arabia. Eur J Med Plants 2012;2:93-112.






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