Anti-Inflammatory Activity of Extraction and Bioaccessible Fraction of Albizia Lebbeck (L.) Benth. on Interleukin-1β-Induced Inflammation in Human Retinal Pigment Epithelial (ARPE-19) Cells
Keywords:
Albizia lebbeck (L.) Benth, Age-related macular degeneration, Inflammation, Interleukin-1β, ARPE-19 cells, ARPE-19 cellsAbstract
Age-related macular degeneration (AMD) is a common eye disease that affects older adults and is the leading cause of vision loss. The major cause of AMD is inflammation of retinal pigment epithelium (RPE) cells. Albizia lebbeck (L.) Benth. leaves contain various phytochemicals that have several biological attributes, especially anti-inflammatory properties. This study examined the anti-inflammatory effect of extraction and bioaccessible fraction of A. lebbeck leaves (ALE and BFA, respectively) on human retinal pigment epithelial ARPE-19 cells that were stimulated for inflammation by interleukin-1β (IL-1β). ARPE-19 cells were pre-treated with ALE or BFA for 1 h before inducing with 0.1 ng/ml IL-1β for another 24 h and then induced with IL-1β for 24 h. Our results show that pretreatment with ALE and BFA significantly reduced the secretion of pro-inflammatory mediators (IL-6, IL-8, and MCP-1) without toxicity (p < 0.05). These findings suggest that ALE can protect human retina cells from inflammatory induction and thus AMD development. The portion of A. lebbeck leaves that underwent simulated digestion retained its ability to protect ARPE-19 cells from inflammatory induction. However, further investigation into the impact of A. lebbeck leaves on AMD is warranted, including studies involving both experimental animal models and human subjects.
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