Protective Effects of Extraction and Bioaccessible Fraction of Mulberry on Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial (ARPE-19) Cells

Authors

  • Kanokkorn Somboon Graduate student in Master of Science Program in Toxicology and Nutrition for Food Safety, Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand
  • Pornpan Sukboon Community Nutrition Unit, Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand
  • Rianthong Phumsuay Cell and Animal Model Unit, Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand
  • Siriporn Tuntipopipat Cell and Animal Model Unit, Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand
  • Monruedee Sukprasansap Food Toxicology Unit, Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand
  • Chawanphat Muangnoi Cell and Animal Model Unit, Institute of Nutrition, Mahidol University

Keywords:

Morus alba L., ARPE-19 cells, Age-related macular degeneration, Oxidative stress, Simulated digestion

Abstract

Age-related macular degeneration (AMD) is a condition affecting the macula in the retina that can lead to vision impairment and blindness. Oxidative stress is an important factor in AMD development. Mulberry (Morus alba L.) is a rich source of anthocyanins, a class of naturally occurring biologically active compounds containing a powerful radical-scavenging antioxidant that can neutralize free radicals. This study investigated the effects of mulberry extract (ME) and bioaccessible fraction of mulberry (BFM) on H2O2-induced oxidative damage in human retinal pigment epithelial (ARPE-19) cells. Cells were pre-treated with ME or BFM for 24 h and then exposed to H2O2 to induce oxidative damage. Results indicated that both ME and BFM significantly (p<0.05) protected ARPE-19 cells from induced oxidative damage by decreasing the level of intracellular reactive oxygen species (ROS). Furthermore, after simulated digestion, mulberry demonstrated a statistically significant capability to mitigate oxidative damage in retinal cells. These findings suggest that both ME and BFM can protect human retina cells from oxidative damage induction and AMD development.  Since this study was limited to in vitro research, further investigations into the impact of mulberry on AMD are warranted encompassing both experimental animal models and human subjects.

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Published

2024-05-24

How to Cite

Somboon, K., Sukboon, P. ., Phumsuay, R., Tuntipopipat, S., Sukprasansap, M., & Muangnoi, C. (2024). Protective Effects of Extraction and Bioaccessible Fraction of Mulberry on Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial (ARPE-19) Cells. Journal of Nutrition Association of Thailand (Online), 59(1), 60–72. Retrieved from https://he01.tci-thaijo.org/index.php/JNAT/article/view/270994

Issue

Vol. 59 No. 1 (2024): January-June

Section

Research article

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Upon acceptance of an article, copyright is belonging to the Nutrition Association of Thailand.