Blood-Based Proteome Profiles among Healthy and Obese Men with Carotenoid-Rich Jelly Consumption


  • Kanda Kaewhom Master of Science Program in Nutrition, Faculty of Medicine Ramathibodi Hospital and Institute of Nutrition, Mahidol University
  • Sittiruk Roytrakul Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency
  • Pornrat Sinchaipanit Institute of Nutrition, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom, Thailand
  • Patcharin Kaewboonruang Faculty of Medicine Ramathibodi Hospital, Mahidol University
  • ๋Jintana Sirivarasai Faculty of Medicine Ramathibodi Hospital, Mahidol University


obesity, carotenoid, serum proteome, plasma β-carotene, molecular pathways


Proteomics has clarified the link between obesity and the development of cardiovascular diseases, which involves a combination of genetic predisposition and environmental factors. This randomized, placebo-controlled study investigated the effects of carotenoid in carrot jelly on protein profiles and the clinical and biochemical markers of metabolic syndrome in adult men with obesity. Thirty participants were divided into three groups: healthy (n = 10), obese-treatment (n = 10), and obese-placebo (n = 10). Daily consumption of 200 mL carrot jelly for 3 months in the treatment group led to significant decreases in low-density lipoprotein-cholesterol, fasting plasma glucose and plasma malondialdehyde, and a significant increase in plasma β-carotene (p < 0.05). After 3 months of supplementation, protein interactions in the treatment group indicated important pathways involving Wnt regulation and cell surface-receptor signaling pathways for the upregulated proteins, as well as regulation of cytokine production and cell proliferation pathways for the downregulated proteins. The proteomic patterns clarified the molecular mechanisms underlying obesity-induced metabolic disorders and the potential benefits of carotenoid consumption. Such studies may critically improve serious nutrition-related health issues.


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Abdelaal M, le Roux CW, Docherty NG. Morbidity and mortality associated with obesity. Ann Transl Med 2017;5(7):161.

Non-communicable diseases: risk factors. In: Global Health Observatory [website]. Geneva: World Health Organization, 2021 (

Bays HE, Toth PP, Kris-Etherton PM, Abate N, Aronne LJ, Brown WV, et al. Obesity, adiposity, and dyslipidemia: a consensus statement from the National Lipid Association. J Clin Lipidol 2013;7(4):304-83.

Klop B, Elte JW, Cabezas MC. Dyslipidemia in obesity: mechanisms and potential targets. Nutrients 2013;5(4):1218-40.

Coronel J, Pinos I, Amengual J. β-carotene in obesity research: technical considerations and current status of the field. Nutrients 2019;11(4):842.

Li R, Hong P, Zheng X. β-carotene attenuates lipopolysaccharide-induced inflammation via inhibition of the NF-κB, JAK2/STAT3 and JNK/p38 MAPK signaling pathways in macrophages. Anim Sci J 2019;90(1):140-8.

Fiedor J, Burda K. Potential role of carotenoids as antioxidants in human health and disease. Nutrients 2014;6(2):466-88.

Choi CI. Astaxanthin as a peroxisome proliferator-activated receptor (PPAR) modulator: its therapeutic implications. Mar Drugs 2019;17(4):242.

Roohbakhsh A, Karimi G, Iranshahi M. Carotenoids in the treatment of diabetes mellitus and its complications: A mechanistic review. Biomed Pharmacother 2017;91:31-42.

Beydoun MA, Chen X, Jha K, Beydoun HA, Zonderman AB, Canas JA. Carotenoids, vitamin A, and their association with the metabolic syndrome: a systematic review and meta-analysis. Nutr Rev 2019;77(1):32-45.

Asemi Z, Alizadeh SA, Ahmad K, Goli M, Esmaillzadeh A. Effects of beta-carotene fortified synbiotic food on metabolic control of patients with type 2 diabetes mellitus: A double-blind randomized cross-over controlled clinical trial. Clin Nutr 2016;35(4):819-25.

Canas JA, Damaso L, Altomare A, Killen K, Hossain J, Balagopal PB. Insulin resistance and adiposity in relation to serum beta-carotene levels. J Pediatr 2012;161:58-64, e1-2.

Canas JA, Lochrie A, McGowan AG, Hossain J, Schettino C, Balagopal PB. Effects of mixed carotenoids on adipokines and abdominal adiposity in children: a pilot study. J Clin Endocrinol Metab 2017;102:1983-90.

Yang J, Zhang Y, Na X, Zhao A. β-carotene supplementation and risk of cardiovascular disease: a systematic review and meta-analysis of randomized controlled trials. Nutrients 2022;14(6):1284.

Zaghlool SB, Sharma S, Molnar M, Matías-García PR, Elhadad MA, Waldenberger M, et al. Revealing the role of the human blood plasma proteome in obesity using genetic drivers. Nat Commun 2021;12(1):1279.

Eroglu A, Schulze KJ, Yager J, Cole RN, Christian P, Nonyane BAS, et al. Plasma proteins associated with circulating carotenoids in Nepalese school-aged children. Arch Biochem Biophys 2018;646:153-60.

Immundiagnostik AG. Malondialdehyde HPLC Kit 2010 [cited 27 September 2019]. Available from:

CHROMSYSTEM. Vitamins A and E in Serum/Plasma – HPLC [cited 16 October 2020]. Available from:

CHROMSYSTEM. β-Carotene in Serum/ Plasma—HPLC [cited 16 October 2020]. Available from:

AOAC. Official Methods of Analysis of AOAC International. 21th ed. Association of Official Analytical Chemists International, Maryland, USA, 2019.

Speek AJ, Temalilwa CR, Schrijver J. Determination of β-carotene content and vitamin A activity of vegetables by high-performance liquid chromatography and spectrophotometry. Food Chem 1986;19(1):65-74.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.

Murad AM, Souza GH, Garcia JS, Rech EL. Detection and expression analysis of recombinant proteins in plant-derived complex mixtures using nanoUPLC-MS(E). J Sep Sci 2011;34(19):2618-30.

Hajizadeh-Sharafabad F, Zahabi ES, Malekahmadi M, Zarrin R, Alizadeh M. Carotenoids supplementation and inflammation: a systematic review and meta-analysis of randomized clinical trials. Crit Rev Food Sci Nutr 2022;62(29):8161-77.

Pérez-Torres I, Castrejón-Téllez V, Soto ME, Rubio-Ruiz ME, Manzano-Pech L, Guarner-Lans V. Oxidative stress, plant natural antioxidants, and obesity. Int J Mol Sci 2021;22(4):1786.

Chisté RC, Freitas M, Mercadante AZ, Fernandes E. Carotenoids inhibit lipid peroxidation and hemoglobin oxidation, but not the depletion of glutathione induced by ROS in human erythrocytes. Life Sci 2014;99(1-2):52-60.

Wang Y, Chung SJ, McCullough ML, Song WO, Fernandez ML, Koo SI, et al. Dietary carotenoids are associated with cardiovascular disease risk biomarkers mediated by serum carotenoid concentrations. J Nutr 2014;144(7):1067-74.

Zhu X, Shi M, Pang H, Cheang I, Zhu Q, Guo Q, et al. Inverse association of serum carotenoid levels with prevalence of hypertension in the general adult population. Front Nutr 2022;9:971879.

Rodrigo R, González J, Paoletto F. The role of oxidative stress in the pathophysiology of hypertension. Hypertens Res 2011;34(4):431-40.

Sindhu S, Thomas R, Shihab P, Sriraman D, Behbehani K, Ahmad R. Obesity is a positive modulator of IL-6r and IL-6 expression in the subcutaneous adipose tissue: significance for metabolic inflammation. PLoS One 2015;10(7):e0133494.

Mahadev K, Motoshima H, Wu X, Ruddy JM, Arnold RS, Cheng G, et al. The NAD(P)H oxidase homolog Nox4 modulates insulin-stimulated generation of H2O2 and plays an integral role in insulin signal transduction. Mol Cell Biol 2004;24(5):1844-54.

Pintus F, Floris G, Rufini A. Nutrient availability links mitochondria, apoptosis, and obesity. Aging (Albany NY) 2012;4(11):734-41.

Kuhajda FP, Aja S, Tu Y, Han WF, Medghalchi SM, El Meskini R, et al. Pharmacological glycerol-3-phosphate acyltransferase inhibition decreases food intake and adiposity and increases insulin sensitivity in diet-induced obesity. Am J Physiol Regul Integr Comp Physiol 2011;301(1):R116-30.

Moreira TG, Zhang L, Shaulov L, Harel A, Kuss SK, Williams J, et al. Sec13 regulates expression of specific immune factors involved in inflammation in vivo. Sci Rep 2015;5:17655.

Ghosh Dastidar S, Jagatheesan G, Haberzettl P, Shah J, Hill BG, Bhatnagar A, et al. Glutathione S-transferase P deficiency induces glucose intolerance via JNK-dependent enhancement of hepatic gluconeogenesis. Am J Physiol Endocrinol Metab 2018;315(5):E1005-18.

Cominetti O, Núñez Galindo A, Corthésy J, Valsesia A, Irincheeva I, Kussmann M, et al. Obesity shows preserved plasma proteome in large independent clinical cohorts. Sci Rep 2018;8(1):16981.

Rosa LRO, Soares GM, Silveira LR, Boschero AC, Barbosa-Sampaio HCL. ARHGAP21 as a master regulator of multiple cellular processes. J Cell Physiol 2018;233(11):8477-81.

Soares GM, Zangerolamo L, Azevedo EG, Costa-Júnior JM, Carneiro EM, Saad ST, et al. Whole body ARHGAP21 reduction improves glucose homeostasis in high-fat diet obese mice. J Cell Physiol 2018;233(9):7112-9.

Axelsson AS, Mahdi T, Nenonen HA, Singh T, Hänzelmann S, Wendt A, et al. Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes. Nat Commun 2017;8:15652.

Ramseyer VD, Kimler VA, Granneman JG. Vacuolar protein sorting 13C is a novel lipid droplet protein that inhibits lipolysis in brown adipocytes. Mol Metab 2018;7:57-70.

Song G, Zhang S, Tian M, Zhang L, Guo R, Zhuo W, et al. Molecular insights into the human ABCB6 transporter. Cell Discov 2021;7(1):55.

Lynch J, Fukuda Y, Krishnamurthy P, Du G, Schuetz JD. Cell survival under stress is enhanced by a mitochondrial ATP-binding cassette transporter that regulates hemoproteins. Cancer Res 2009;69:5560-7.

McPherson LA, Loktev AV, Weigel RJ. Tumor suppressor activity of AP2alpha mediated through a direct interaction with p53. J Biol Chem 2002;277(47):45028-33.

Lee YK, Chung Y, Lee JH, Chun JM, Park JH. The intricate role of p53 in adipocyte differentiation and function. Cells 2020;9(12):2621.

González-Domínguez Á, Visiedo-García FM, Domínguez-Riscart J, González-Domínguez R, Mateos RM, Lechuga-Sancho AM. Iron metabolism in obesity and metabolic syndrome. Int J Mol Sci 2020;21(15):5529.

Goddi A, Schroedl L, Brey EM, Cohen RN. Laminins in metabolic tissues. Metabolism 2021;120:154775.

Gonzalez Porras MA, Stojkova K, Vaicik MK, Pelowe A, Goddi A, Carmona A, et al. Integrins and extracellular matrix proteins modulate adipocyte thermogenic capacity. Sci Rep 2021;11(1):5442.

Choi MJ, Mukherjee S, Yun JW. Loss of ADAMTS15 promotes browning in 3T3-L1 white adipocytes via activation of β3-adrenergic receptor. Biotechnol Bioprocess Eng 2021;26:188-200.

Zamponi GW, Striessnig J, Koschak A, Dolphin AC. The physiology, pathology, and pharmacology of voltage-gated calcium channels and their future therapeutic potential. Pharmacol Rev 2015;67(4):821-70.

Actis Dato V, Chiabrando GA. The role of low-density lipoprotein receptor-related protein 1 in lipid metabolism, glucose homeostasis and inflammation. Int J Mol Sci 2018;19(6):1780.

Buchner DA, Charrier A, Srinivasan E, Wang L, Paulsen MT, Ljungman M, et al. Zinc finger protein 407 (ZFP407) regulates insulin-stimulated glucose uptake and glucose transporter 4 (Glut4) mRNA. J Biol Chem 2015;290(10):6376-86.

Park HS, Ju UI, Park JW, Song JY, Shin DH, Lee KH et al. PPARγ neddylation essential for adipogenesis is a potential target for treating obesity. Cell Death Differ 2016;23:1296-311.

Hall JE. The kidney, hypertension, and obesity. Hypertension. 2003;41(3 Pt 2):625-33.

Park HA, Hayden MM, Bannerman S, Jansen J, Crowe-White KM. Anti-apoptotic effects of carotenoids in neurodegeneration. Molecules 2020;25(15):3453.

Coletta DK, Mandarino LJ. Mitochondrial dysfunction and insulin resistance from the outside in: extracellular matrix, the cytoskeleton, and mitochondria. Am J Physiol Endocrinol Metab 2011;301(5):E749-55.

Williams AS, Kang L, Wasserman DH. The extracellular matrix and insulin resistance. Trends Endocrinol Metab 2015;26(7):357-66.

Ortiz C, Schierwagen R, Schaefer L, Klein S, Trepat X, Trebicka J. Extracellular matrix remodeling in chronic liver disease. Curr Tissue Microenviron Rep 2021;2(3):41-52.

Wai Wong C, Dye DE, Coombe DR. The role of immunoglobulin superfamily cell adhesion molecules in cancer metastasis. Int J Cell Biol 2012;2012:340296.

Lewitt MS, Dent MS, Hall K. The insulin-like growth factor system in obesity, insulin resistance and type 2 diabetes mellitus. J Clin Med 2014;3(4):1561-74.

Bender T, Martinou JC. The mitochondrial pyruvate carrier in health and disease: To carry or not to carry? Biochim Biophys Acta 2016;1863(10):2436-42.

Møller LLV, Klip A, Sylow L. Rho GTPases-emerging regulators of glucose homeostasis and metabolic health. Cells 2019;8(5):434.

Chen N, Wang J. Wnt/β-catenin signaling and obesity. Front Physiol 2018;9:792.

Gilbert SF. Developmental Biology. 6th edition. Sunderland (MA): Sinauer Associates; 2000. Cell Surface Receptors and Their Signal Transduction Pathways. Available from:

Kaulmann A, Bohn T. Carotenoids, inflammation, and oxidative stress— implications of cellular signaling pathways and relation to chronic disease prevention. Nutr Res 2014;34(11):907-29.

Gopal SS, Eligar SM, Vallikannan B, Ponesakki G. Inhibitory efficacy of lutein on adipogenesis is associated with blockage of early phase regulators of adipocyte differentiation. Biochim Biophys Acta Mol Cell Biol Lipids 2021;1866(1):158812.




How to Cite

Kaewhom, K. ., Roytrakul, S. ., Sinchaipanit, P. ., Kaewboonruang, P. ., & Sirivarasai ๋. (2023). Blood-Based Proteome Profiles among Healthy and Obese Men with Carotenoid-Rich Jelly Consumption. Journal of Nutrition Association of Thailand, 58(1), 68–90. Retrieved from



Research article