Gac Fruit Extract Suppresses Oxidative Stress in LPS-induced RAW264.7 Cells
Keywords:
Gac fruit, Antioxidant enzyme, Oxidative stressAbstract
Gac fruit (Momordica cochinchinensis) contains various bioactive compounds that possess several health benefits, including antioxidant activity. The present study assessed the antioxidant effects of ethanol-extract from ripe Gac fruit on LPS-induced murine macrophage cell line (RAW 264.7 cell). Pulp and aril from ripe Gac fruit were collected separately, homogenized, and lyophilized. An equal weight of dry powder pulp and aril were homogeneously mixed (Gac fruit powder). The ethanol extract of Gac fruit powder had b-carotene and lycopene of 206 and 231 mg/g dry weight (DW), respectively; total flavonoids of 1.5 mg Quercetin Equivalent (QE)/g DW; and phenolic compounds of 3.0 mg Gallic Acid Equivalent (GAE)/g DW. RAW264.7 cells significantly generated reactive oxygen species (ROS) once exposed to Lipopolysaccharides (LPS). Treatment RAW264.7 cells with 0.5 – 2.0 mg/mL of Gac fruit powder ethanol-extract significantly suppressed LPS-induced intracellular ROS formation in a concentration-dependent manner. Treatment with the extract protected the LPS activated stressed cells by significantly restoring total glutathione content, superoxide dismutase, and catalase activities in LPS-induced RAW264.7 cells. These results indicate that Gac fruit ethanol-extract exhibited moderate antioxidant effect in this cell model.
References
Kubola J, Siriamornpun S. Phytochemicals and antioxidant activity of different fruit fractions (peel, pulp, aril and seed) of Thai gac (Momordica cochinchinensis Spreng). Food Chem. 2011; 127(3):1138-45.
Kubola J, Meeso N, Siriamornpun S. Lycopene and beta carotene concentration in aril oil of gac (Momordica cochinchinensis Spreng) as influenced by aril-drying process and solvents extraction. Food Res Int. 2013; 50(2):664-9.
Le Khac Lam Dien, Nguyen Phuoc Minh, Dong Thi Anh Dao. Investigation different pretreatment methods and ratio of carrier materials to maintain carotenoids in Gac (Momordica Cochinchinensis Spreng) powder in drying process. IJSTR. 2013; 2(12):360-71.
Sukboon P, Tuntipopipat S, Praengam K. Ethanol extract of aril and pulp from Momordica cochinchinensis fruit exhibits antiinflammatory effect in LPS-induced macrophage RAW264.7 cells. Thai J Toxicol. 2019; 34(1):71-90.
Saini RK, Nile SH, Park SW. Carotenoids from fruits and vegetables: Chemistry, analysis, occurrence, bioavailability and biological activities. Food Res Int. 2015; 76:735-50.
Rafi MM, Yadav PN, Reyes M. Lycopene inhibits LPS-Induced proinflammatory mediator inducible nitric oxide synthase in mouse macrophage cells. J Food Sci. 2007; 72(1):S069-S74.
Bai S-K, Lee S-J, Na H-J, Ha K-S, Han J-A, Lee H, et al. -Carotene inhibits inflammatory gene expression in lipopolysaccharide-stimulated macrophages by suppressing redox-based NF-B activation. Exp Mol Med. 2005; 37(4):323-34.
Mengoni ES, Vichera G, Rigano LA, Rodriguez-Puebla ML, Galliano SR, Cafferata EE, et al. Suppression of COX-2, IL-1B and TNF-α expression and leukocyte infiltration in inflamed skin by bioactive compounds from Rosmarinus officinalis L. Fitoterapia. 2011; 82(3):414-21.
Pendyala S, Natarajan V. Redox regulation of Nox proteins. Respir Physiol Neurobiol. 2010; 174(3):265-71.
Thannickal VJ, Fanburg BL. Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol. 2000; 279(6):1005-28.
Dröge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002; 82(1):47-95.
Griffith B, Pendyala S, Hecker L, Lee PJ, Natarajan V, Thannickal VJ. NOX enzymes and pulmonary disease. Antioxid Redox signal. 2009; 11(10):2505-16.
Richardson A, Schadt E. The role of macromolecular damage in aging and age-related dsease. J Gerontol A Bio Sci Med Sci. 2014; 69 Suppl 1:S28-32.
Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB. Reactive oxygen species in inflammation and tissue injury. Antioxid Redox signal. 2014; 20(7):1126-67.
Edge R, McGarvey DJ, Truscott TG. The carotenoids as anti-oxidants - a review. J Photochem Photobiol B Biol. 1997; 41(3):189-200.
Kaurinovic B, Vastag G. Flavonoids and phenolic acids as potential natural antioxidants. 2019. doi/10.5772/intechopen. 83731.
Rafat Husain S, Cillard J, Cillard P. Hydroxyl radical scavenging activity of flavonoids. Phytochemistry. 1987; 26(9):2489-91.
Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol. 1988; 37(5):837-41.
Jan-on G, Pakdeechote P, Kukongviriyapan V, Kongsaktrakoon B, Boonla O, Kukongviriyapan U. Alleviation of hypertension and oxidative stress by Momordica cochinchinensis aril extract in rats with nitric oxide deficiency. Srinagarind Med J. 2015; 30(3):229-35.
Failla ML, Chitchumroonchokchai C, Ishida BK. In vitro micellarization and intestinal cell uptake of cis isomers of lycopene exceed those of all-trans lycopene. J Nutr. 2008; 138(3):482-6.
Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nat Protocol. 2007; 2(4):875-7.
Bag GC, Devi Grihanjali P, Bhaigyabati T. Assessment of total flavonoid content and antioxidant activity of methanolic rhizome extract of three Hedychium species of Manipur valley. Int J Pharm Sci Rev Res. 2015; 30(1):154-9.
Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protocol. 2006; 1(3):1112-6.
Zhang S, Wang P, Zhao P, Wang D, Zhang Y, Wang J, et al. Pretreatment of ferulic acid attenuates inflammation and oxidative stress in a rat model of lipopolysaccharide-induced acute respiratory distress syndrome. Int J Immunopathol Pharmacol. 2018; 32: 394632017750518.
Saokosol Y, Praengam K, Sukprasansap M, Tuntipopipat S. Antioxidant potential of ethanol extract from orange fleshed sweet potato (Ipomoea batatas) in murine macrophage RAW264.7 cell line. J Nutr Assoc Thailand. 2019; 54(1):37-54.
Rahman I, Kode A, Biswas SK. Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. Nat Protocol. 2006; 1(6):3159-65.
Vandeputte C, Guizon I, Genestie-Denis I, Vannier B, Lorenzon G. A microtiter plate assay for total glutathione and glutathione disulfide contents in cultured/isolated cells: performance study of a new miniaturized protocol. Cell Biol Toxicol. 1994; 10(5-6):415-21.
Pinthong S, Kunchit J, Tangsuphoom N, Jittinandana S and and Nakngamanong Y. Effect of different drying processes on physical properties and carotenoid content of Gac fruit (Momordica cochinchinensis Spreng). J Food Sci Agr Tech. 2019; 5:61-70.
Kubola J, Meeso N and Siriamornpun S. Lycopene and beta carotene concentration in aril oil of gac (Momordica cochinchinensis Spreng) as influenced by aril-drying process and solvents extraction. Food Res Int. 2013; 50(2);664-669.
Abdulqader A, Ali F, Ismail A, Esa N. Antioxidant compounds and capacities of Gac (Momordica cochinchinensis Spreng) fruits. Asian Pac J of Trop Biomed. 2019; 9(4):158-67.
Khan M, Qurashi N, Khan M, Jabeen F, Umar A, Yaqoob J, et al. Generation of reactive oxygen species and their impact on the health related parameters: A critical review. Int J Biosci. 2016; 9:303-23.
Halliwell B, Gutteridge JMC. Role of free radicals and catalytic metal ions in human disease: An overview. Methods Enzymol. 1990; 186:1-85.
Bai S-K, Lee S-J, Na H-J, Ha K-S, Han J-A, Lee H, et al. Beta-Carotene inhibits inflammatory gene expression in lipopolysaccharide-stimulated macrophages by suppressing redox-based NF-kappa-B activation. Exp Mol Med. 2005; 37:323-34.
Zou J, Feng D, Ling WH, Duan RD. Lycopene suppresses proinflammatory response in lipopolysaccharide-stimulated macrophages by inhibiting ROS-induced trafficking of TLR4 to lipid raft-like domains. J Nutr Biochem. 2013; 24(6):1117-22.
Manuja R, Sachdeva S, Jain A, Chaudhary J. A Comprehensive review on biological activities of p-hydroxy benzoic acid and its derivatives. Int J Pharm Sci Rev Res. 2013; 22(2):109-15.
Bouzaiene N, Jaziri S, Kovacic H, Chekir-Ghedira L, Luis J. The effects of caffeic, coumaric and ferulic acids on proliferation, superoxide production, adhesion and migration of human tumor cells in vitro. Eur J Pharmacol. 2015; 766:99-105.
Semwal D, Semwal R, Combrinck S, Viljoen A. Myricetin: A Dietary Molecule with Diverse Biological Activities. Nutrients. 2016; 8(90):1-31.
Ali F, Rahul, Naz F, Jyoti S, Siddique Y. Health Functionality of Apigenin: A Review. Int J Food Prop. 2017; 20(6):1197-238.
Lin W-T, Huang C-C, Lin T-J, Chen J, Shieh M-J, Peng H-C, et al. Effects of B-carotene on antioxidant status in rats with chronic alcohol consumption. Cell Biochem Funct. 2009; 27:344-50.
Joe B, Lokesh BR. Studies on the inactivation of superoxide dismutase activity by nitric oxide from rat peritoneal macrophages. Mol Cell Biochem. 1997; 168(1):87-93.
Dai C, Tang S, Deng S, Zhang S, Zhou Y, Velkov T, et al. Lycopene attenuates colistin-induced nephrotoxicity in mice via activation of the Nrf2/HO-1 pathway. Antimicrob Agents Chemother. 2015; 59(1):579.
Morakinyo F, Bolanle I, Oyelowo O, Nnaji J. Anti-oxidative and hepatoprotective effect of beta-carotene on acetaminophen-induced liver damage in rats. Biol Med. 2012; 4(3):134-40.
Hosseini F, Naseri MKG, Badavi M, Ghaffari MA, Shahbazian H, Rashidi I. Effect of beta carotene on lipid peroxidation and antioxidant status following renal ischemia/reperfusion injury in rat. Scand J Clin Lab Invest. 2010; 70(4):259-63.
Sheik Abdulazeez S, Thiruvengadam D. Effect of lycopene on oxidative stress induced during d-galactosamine/ lipopolysaccharide-sensitized liver injury in rats. Pharmaceut Biol. 2013; 51(12):1592-9.
Bose KSC, Agrawal BK. Effect of lycopene from tomatoes (cooked) on plasma antioxidant enzymes, lipid peroxidation rate and lipid profile in grade-I hypertension. Ann Nutr Metab. 2007; 51(5):477-81.
Bose KS, Agrawal BK. Effect of lycopene from cooked tomatoes on serum antioxidant enzymes, lipid peroxidation rate and lipid profile in coronary heart disease. Singapore Med J. 2007; 48(5):415-20.
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