Gac Fruit Extract Suppresses Oxidative Stress in LPS-induced RAW264.7 Cells
Keywords:Gac fruit, Antioxidant enzyme, Oxidative stress
Gac fruit (Momordica cochinchinensis) contains various bioactive compounds that possess several health benefits, including antioxidant activity. The present study assessed the antioxidant effects of ethanol-extract from ripe Gac fruit on LPS-induced murine macrophage cell line (RAW 264.7 cell). Pulp and aril from ripe Gac fruit were collected separately, homogenized, and lyophilized. An equal weight of dry powder pulp and aril were homogeneously mixed (Gac fruit powder). The ethanol extract of Gac fruit powder had b-carotene and lycopene of 206 and 231 mg/g dry weight (DW), respectively; total flavonoids of 1.5 mg Quercetin Equivalent (QE)/g DW; and phenolic compounds of 3.0 mg Gallic Acid Equivalent (GAE)/g DW. RAW264.7 cells significantly generated reactive oxygen species (ROS) once exposed to Lipopolysaccharides (LPS). Treatment RAW264.7 cells with 0.5 – 2.0 mg/mL of Gac fruit powder ethanol-extract significantly suppressed LPS-induced intracellular ROS formation in a concentration-dependent manner. Treatment with the extract protected the LPS activated stressed cells by significantly restoring total glutathione content, superoxide dismutase, and catalase activities in LPS-induced RAW264.7 cells. These results indicate that Gac fruit ethanol-extract exhibited moderate antioxidant effect in this cell model.
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