Water extract from leaf and stem of White Mugwort inhibits enzyme activity of α-amylase and α-glucosidase
Keywords:Type 2 Diabetes, White Mugwort (Artemisia lactiflora), Alpha-amylase
Type 2 diabetes (T2D) is the condition that a person has high blood glucose. This condition results from insulin insensitivity or the lack of insulin. T2D is one of global public health problems including Thailand. So far, many studies have reported anti-diabetic activities of various plant extracts. This research focused on the inhibitory potential of aqueous extract from leaf and stem of White Mugwort (Artemisia lactiflora) on digestive enzymes related to type 2 diabetes. The study found that total phenolic content of aqueous White Mugwort extract was 1.25 mggallic acid equivalent/g dry weight. The aqueous White Mugwort extract also shows the inhibitory effect ona-amylase with IC50 = 44.2 mg/mL, anda-glucosidase with IC50 = 1.3 mg/mL. This is the first time that the inhibitory effect of White Mugwort against carbohydrate-metabolizing enzymes is reported. Overall, our findings suggest that regular consumption of White Mugwort may slow release of simple sugars in the gut and thereby decrease postprandial hyperglycemia.
2. Sae-tan S, Grove KA, Lambert JD. Weight control and prevention of metabolic syndrome by green tea. Pharmacol Res. 2011;64:146-54.
3. Deerochanawong C, Ferrario A. Diabetes management in Thailand: a literature review of the burden, costs, and outcomes. Globalization and Health.
4. Chao PY, Lin SY, Lin KH, Liu YF, Hsu JI, Yang CM, et al. Antioxidant activity in extracts of 27 indigenous Taiwanese vegetables. Nutrients. 2014;6:2115-30.
5. Nabavi SF, Russo GL, Daglia M, Nabavi SM. Role of quercetin as an alternative for obesity treatment: you are what you eat! Food Chemistry. 2015;179:305-10.
6. Prahalathan P, Kumar S, Raja B. Effect of morin, a flavonoid against DOCA-salt hypertensive rats: a dose dependent study. Asian Pacific Journal of Tropical Biomedicine. 2012;2:443-8.
7. Paoli P, Cirri P, Caselli A, Ranaldi F, Bruschi G, Santi A, et al. The insulin-mimetic effect of Morin: a promising molecule in diabetes treatment. Biochim Biophys Acta. 2013;1830:3102-11.
8. Vanitha P, Uma C, Suganya N, Bhakkiyalakshmi E, Suriyanarayanan S, Gunasekaran P, et al. Modulatory effects of morin on hyperglycemia by attenuating the hepatic key enzymes of carbohydrate metabolism and β-cell function in streptozotocin-induced diabetic rats. Environmental Toxicology and Pharmacology. 2014;37:326-35.
9. Li YQ, Zhou FC, Gao F, Bian JS, Shan F. Comparative evaluation of quercetin, isoquercetin and rutin as inhibitors of alpha-glucosidase. J Agric Food Chem. 2009;57:11463-8.
10. Farsi E, Shafaei A, Hor SY, Khadeer Ahamed MB, Yam MF, Attitalla IH, et al. Correlation between enzymes inhibitory effects and antioxidant activities of standardized fractions of methanolic extract obtained from Ficus deltoidea leaves. African Journal of Biotechnology. 2011;10:15184-94.
11. Oboh G, Ogunsuyi OB, Ogunbadejo MD, Adefegha SA. Influence of gallic acid on α-amylase and α-glucosidase inhibitory properties of acarbose. Journal of Food and Drug Analysis 2016;24:627-34.
12. Lordan S, Smyth TJ, Soler-Vila A, Stanton C, Ross RP. The α-amylase and α-glucosidase inhibitory effects of Irish seaweed extracts. Food Chemistry 2013;141:2170-6.
13. Adisakwattana S, Ruengsamran T, Kampa P, Sompong W. In vitro inhibitory effects of plant-based foods and their combinations on intestinal α-glucosidase and pancreatic α-amylase. BMC Complementary and Alternative Medicine 2012;12:110.
14. Spinola V, Castilho PC. Evaluation of Asteraceae herbal extracts in the management of diabetes and obesity: Contribution of caffeoylquinic acids on the inhibition of digestive enzymes activity and formation of advanced glycation end-products (in vitro). Phytochemistry 2017;143:29-35.
15. Etxeberria U, de la Garza AL, Campion J, Martinez JA, Milagro FI. Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylase. Expert Opin Ther Tar 2012;16:269-97.
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