Preliminary Study : Prevalence of ESBL-Producing Enterobacterales in Cockroaches’ intestine among Community Housing, Samut Prakan Province
Keywords:
American cockroach, prevalence, ESBL-producing EnterobacteralesAbstract
This preliminary study aimed to survey the prevalence of extended-spectrum b-lactamase (ESBL)-producing Enterobacterales from cockroach intestines. A total of 55 cockroaches were collected from households in community housing, Samut Prakan province and further identified. All guts were cultured on selective and differential agar for bacterial isolation. The bacterial species were identified by using biochemical media or MALDI-TOF Mass Spectrometry and further identified for ESBL production by phenotypic and genotypic methods. The result showed that all cockroaches were American cockroaches (Periplaneta americana). Medically significant bacteria were isolated from the intestines of 100% of cockroaches. The top three bacterial species identified were Citrobacter freundii, Escherichia coli, and Klebsiella pneumoniae (30.67, 17.33, and 12.0%, respectively). ESBL expression was detected in one strain of C. freundii and confirmed to be blaVEB by PCR, representing a prevalence of 1.33%. In conclusion, P. americana is considered a potential carrier and a vector of pathogenic bacteria, especially transmission of ESBL-producing Enterobacterales.
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