Antibacterial Efficacies of Leaf Extract of Millingtonia hortensis Linn on Streptococcus mutans.

Authors

  • Ladda Trisupatsil 614 ถ.เตชะวนิช บางซื่อ กทม.

Keywords:

Millingtonia hortensis, Streptococcus mutans, agar disc diffusion

Abstract

Abstract

The purpose of this paper was to study the antibacterial efficacy and determine the minimal inhibitory concentration of Millingtonia hortensis leaf extract on Streptococcus mutans DMST 18777. The research model was an experimental design. Millingtonia hortensis leaves were extracted with 95% ethanol to assess the antibacterial activity with agar disc diffusion method. The minimal inhibitory concentration of Millingtonia hortensis leaf extract was examined by agar dilution method. Statistical analysis was performed by one-way analysis of variance, with the level of significance of 0.01.

We found that Millingtonia hortensis leaf extract was able to inhibit Streptococcus mutans DMST 18777. The inhibition zone diameter was 11.87 mm. Minimal inhibitory concentration of Millingtonia hortensis leaf extract was 40 mg/mL.

References

Petersen PE, Kwan S. Equity, social determinants and public health programmes – the case of oral health. Community Dent Oral Epidemiol. 2011;39(6):481–7.

Petersen PE, Bourgeois D, Ogawa H, Estupinan-Day S, Ndiaye C. The global burden of oral diseases and risks to oral health. Bull World Health Organ. 2005;83(9):661-9.

Rautemaa R, Lauhio A, Cullinan MP, Seymour GJ. Oral infections and systemic disease-an emerging problem in medicine. Clin Microbiol Infect. 2007;13(11):1041-7.

Srisilapanan P, Nirunsittirat A, Roseman J. Trends over time in dental caries status in urban and rural Thai children. J Clin Exp Dent. 2017;19(10): e1201-6.

จิราพร ขีดดี, บรรณาธิการ. รายงานผลการสำรวจสภาวะสุขภาพช่องปากแห่งชาติ ครั้งที่ 8 ประเทศไทย พ.ศ 2560. พิมพ์ครั้งที่ 1. กรุงเทพมหานคร. สามเจริญพาณิชย์; 2561.

Lapirattanakul J. Serotypes of Streptococcus mutans. J Dent Assoc Thai. 2009;59(4):222-32.

Tichy J, Novak J. Extraction, assay, and analysis of antimicrobials from plants with activity against dental pathogens (Streptococcus sp.). J Altern Complement Med. 1998;4:39-45.

Badria FA, Zidan OA. Natural products for dental caries prevention. J Med Food. 2004;7:381-4.

Chung JY, Choo JH, Lee MH, Hwang JK. Anticariogenic activity of macelignan isolated from Myristica fragrans (nutmeg) against Streptococcus mutans. Phytomedicine. 2006;13:261-6.

McCullough MJ, Farah CS. The role of alcohol in oral carcinogenesis with particular reference to alcohol-containing mouthwashes. Aust Dent J. 2008;53:302-5.

Kumari A, Sharma R.A. A Review on Millingtonia hortensis Linn. Int J Pharm Sci Rev Res. 2013; 17:85-92.

Jetty A, Iyengar DS. Antimicrobial activity of Millingtonia hortensis leaf extract. Pharm Biol. 2000;38(2):157-60.

Banavar RS, Nirupad S, Chippagiri P, Pandurangappa R. Antibacterial effects of natural herbal extracts on Streptococcus mutans: can they be potential additives in dentifrices? Int J Dent. 2017;2017:492164.

Chiewpattanakul P, Thongsong N, Suk-arj P, Kitisriworapan W. The Study of the efficiency of 5 herbal crude plant extracts and 0.12% chlorhexidine effecting to antimicrobial activity against Streptococcus mutans. SWU Dent J. 2014;7(2):76-89.

ปัทมาวดี เสตะกัณณะ, จารีย์ บันสิทธิ์, ธิดารัตน์ บุญรอด. การทดสอบฤทธิ์ต้านจุลชีพของสมุนไพรไทย. วารสารกรมวิทยาศาสตร์การแพทย์. 2545;44(2):110-124.

Kraivaphan P, Amornchat C, Triratana T, Tandhachoon K, Soo-ampon S. The testing of Acacia catechu sequential extracts on Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Streptococcus mutans. J Dent Assoc Thai. 1993;43(4):227–33.

Sutter VL, Citron DM, Finegold SM. Agar diffusion (disc) test: Susceptibility testing of anaerobes In: Hoffman S, editor. Wadsworth anaerobic bacteriology manual. 3rd ed. Belmont: Star Publishing Company; 1980. pp.76.

National Committee for Clinical and Laboratory Standards. Performance standards for antimicrobial disk susceptibility tests: approved standards. 9th ed. NCCLS document M2-A9. Wayne, PA: National Committee for Clinical and Laboratory Standards; 2006:1-33.

Balakrishna T, Vidyadhara S, Sasidhar R, Ruchitha B, Prathyusha E. A review on extraction techniques. IAJPS. 2016;3(8):880-91.

Sutter VL, Citron DM, Edelstein MAC, Finegold SM. Agar dilution test : Susceptibility testing of anaerobes In: Hoffman S, editor. Wadsworth anaerobic bacteriology manual. 4th ed. Belmont: Star Publishing Company; 1985. pp.85-90.

Islam MA, Alam MM, Choudhury ME, Kobayashi N, Ahmed MU. Determination of minimum inhibitory concentration (mic) of cloxacillin for selected isolates of methicillin-resistant Staphylococcus aureus (mrsa) with their antibiogram. Bangl J Vet Med. 2008;6(1):121-6.

Agah S, Fereshtehnejad S-M, Yousefnejad A, Siavashi F. Antimicrobial activity of ketoconazole and fluconazole against metronidazole resistance strains of Helicobacter pylori. IJPT. 2008;7(1):57-60.

Khan AU, Sultan A, Tyagi A, Zahoor S, Akram M, Kaur S, et al. Amplification of mecA gene in multi-drug resistant Staphylococcus aureus strains from hospital personnel. J Infect Dev Ctries. 2007;1(3):289-95.

Janaki A, Kaleena PK, Elumalai D, Hemalatha P, Babu M, Velu K, et al. Phytochemical screening, antioxidant and antibacterial activities of Millingtonia hortensis (L). Int J Curr Pharm Res. 2017;9(5):162-7.

Saraya S, Kanta J, Sarisuta N, Temsiririrkkul R, Suvathi Y, Samranrit K, et al. Development of guava extract chewable tablets for anticariogenic activity against Streptococcus mutans. Pharm Sci Asia. 2008;35(1):18-23.

Wongkham S, Laupattarakasaem P, Pienthaweechai K, Areejitranusorn P, Wongkham C, Techanitiswad T. Antimicrobial activity of Streblus asper leaf extract. Phytother Res. 2001;15:119-21.

Saladyanant T, Hongprasong N, Pongsamart S, Apinhasmit W, Thunyakitpisal P. Polysaccharide gel from durian fruit-hull extracts: antibacterial activity against Streptococcus mutans and Aggregatibacter actinomycetemcomitans. CU Dent J. 2007;30:235-44.

Torrungruang K, Vichienroj P, Chutimaworapan S. Antibacterial activity of mangosteen pericarp extract against cariogenic Streptococcus mutans. CU Dent J. 2007;30:1-10.

National Committee for Clinical and Laboratory Standards. Performance standards for antimicrobial susceptibility testing : approved standards. 27th ed. NCCLS document M100. Wayne, PA: National Committee for Clinical and Laboratory Standards; 2017:85.

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Published

2020-12-30

How to Cite

1.
Trisupatsil L. Antibacterial Efficacies of Leaf Extract of Millingtonia hortensis Linn on Streptococcus mutans. HCUJOURNAL [Internet]. 2020 Dec. 30 [cited 2024 Apr. 28];24(2):186-9. Available from: https://he01.tci-thaijo.org/index.php/HCUJOURNAL/article/view/242089

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Research article