Preparation and Characterization of Liposomes Containing Cinnamon and Cajuput Essential Oils
Keywords:
liposomes, cinnamon oil, cajuput oil, encapsulation efficiency, GC-MSAbstract
Abstract
The aim of the study was to prepare liposomes entrapping essential oils in order to decrease volatility and enhance stability. The combination of cinnamon (Cinnamomum burmanii) and cajuput (Melaleuca Leucadendron) oils (1:1 by volume) was selected for incorporation into liposome and was analyzed by a developed gas chromatography-mass spectrometry (GC-MS) method for two major compounds, cinnamaldehyde and eucalyptol. The liposomal formulations composed of phospholipid, cholesterol and oils mixture (2 mg/mL) were prepared using a thin film hydration method. The effects of different mass ratios of phospholipid/cholesterol (60:40, 70:30, 80:20, 90:10) and sonication time (0, 2 and 5 minutes) on oil entrapment efficiency were evaluated. The optimized formulation, containing the mass of phospholipid to cholesterol ratio of 80:20 and generated via sonication for 2 minutes, emerged the maximum amount of the two major components (88.40±0.22%) and was used for further preliminary stability studies at 4+2ºC and the room temperature, 25+2ºC, for a period of 2 months. Results showed that the essential oils in liposomal formulation were quite stable within 2 months at both storage temperatures and had good physical appearance. The percentage of oil retention in the liposomal formulations decreased by 8.78 and 12.83 at both temperatures, respectively. Findings suggested that the combination of cinnamon and cajuput oils which enhance antiacne-inducing bacterial activities and are stabilized in the liposomal formulation could be further developed to provide the anti-acne cosmetic products.
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