Antimicrobial Activity of Endophytic Fungi Isolated From Fagraea fragrans Roxb.

  • Praphaiphit Insain praphaiphit@eau.ac.th
  • Teerawat Boonsom
  • Kalaya Pharadai
  • Nongluksna Sriubolmas
Keywords: Endophytic fungi, Fagraea fragrans Roxb., antimicrobial activity, dual culture agar diffusion

Abstract

The aim of this study was to isolate endophytic fungi which produced secondary metabolites from any part of Fagraea fragrans Roxb. Parts of plants sample (leave, bark and cambium) were surface-sterilized and cultured on 6 mycological media including Czapek yeast autolysate agar (CzYA), malt Czapek agar (MCzA), malt extract agar (MEA), potato dextrose agar (PDA), Sabouraud's dextrose agar (SDA) and yeast extract sucrose agar (YSA) for 14 days. The endophytic fungi were identified based on the microscopic morphology and screened for anti-bacterial and anti-fungi activities by dual culture agar diffusion assay. A total of 24 endophytic fungal isolates were isolated from leaves, bark and cambium of the plant sample. They demonstrated 3 active isolates (12.5%) which showed antimicrobial activities. The isolate Ffra L24 was found to inhibit methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E.coli). The Isolates Ffra B14 and Ffra B15 exhibited synergistic activity with ketoconazole against Candida albicans. The type of culture media affected the bioactive compounds production of endophytic fungi. CzYA, MCzA and YSA were suitable for endophytic fungi to produce bioactive compounds. Classification of Ffra B14, Ffra B15 and Ffra L24 based on the microscopic morphology suggested that they were Coelomycetes.

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References

1. Jones, KE, Patel, NG, Levy MA, Storeygard A, Balk D, Gittleman JL, Daszak P. Global trends in emerging infectious diseases. Nature 2008; 451(7181): 990-993.
2. Tan RX, & Zou WX. Endophytes: a rich source of functional metabolites. Natural product reports 2001; 18(4): 448-459.
3. Vandamme, EJ. The search for novel microbial fine chemicals, agrochemicals and biopharmaceuticals. Journal of biotechnology 1994; 37(2): 89-108.
4. Phitchanuwat P, Wiyakrutta S, Triboun P, Pharadai K, Mondranondra I, Rangkaew N, Sriubolmas N. Anti-MRSA and anti-candida albicans activity of endophytic fungi isolated from Desmodium gangeticum (L.) DC. and Ruellia kerrii Craib. In : proceeding The 39th Congress on Science and Technology of Thailand (Full paper). October 21-23, 2013. Bangkok.
5. Insain P. Bioactive compounds from endophytic fungi. EAU Heritage (Science and Technology) 2017; 11(2): 26-39.
6. de Barros BS, da Silva JP, de Souza Ferro JN, Agra IKR, de Almeida Brito F, Albuquerque ED, Barreto E. Methanol extract from mycelium of endophytic fungus Rhizoctonia sp. induces antinociceptive and anti-inflammatory activities in mice. Journal of natural medicines 2011; 65(3-4): 526-531.
7. Bacon CW, & White JF. Physiological adaptations in the evolution of endophytism in the Clavicipitaceae. In Charies WB, and James FW, Microbial Endophytes. New York: Marcel Dekker Inc., CRC Press. 2000; 251-276.
8. Borges WS, Borges KB, Bonato PS, Said S, Pupo MT. Endophytic fungi: natural products, enzymes and biotransformation. Current Organic Chememistry 2009; 13: 1137–1163.
9. Petrini O. Fungal endophytes of tree leaves. In Andrews JH, and Hirano S. (eds). Microbial Ecology of Leaves. Springer-Verlag, New York: 179-19; 1991.
10. Wiyakrutta S., Sriubolmas N., Panphut W., Thongon N., Danwisetkanjana K., Reungrungsi N., and Meevootisom V. Endophytic fungi with anti-microbial, anti-cancer and anti-malarial activities isolated from Thai medicinal plants. World journal of microbiology and biotechnology 2004; 20(3): 265-272.
11. ราชบัณฑิตยสถาน. (2546). อนุกรมวิธานพืช อักษร ก. กรุงเทพมหานคร: หจก. อรุณการพิมพ์.
12. Griffin O. and Parnell J. Loganiaceae. In Flora of Thailand 1997; 6(3): 198-199. Concise Encyclopedia of Plants in Thailand [cited 2019 Apirl 15]. Avialiable from : http://www.dnp.go.th/botany/detail.aspx?wordsLinkno=664&words=%E0%B8%81%E0%B8%B1%E0%B8%99%E0%B9%80%E0%B8%81%E0%B8%A3%E0%B8%B2&typeword=word
13. Apisantiyakom S. Isolation and identification of bioactive chemical constituents of the roots of Bauhinia saccocalyx Pierre and Fagraea fragrans Roxb. Journal of Science Ladkrabang, 2013; 22(2): 68-83.
14. Bangprapai A, Thongphasuk P and Songsak T. Determination of swertiamarin content by TLC-densitometer in Fragraea fragrans Roxb. leaves. Bulletin of Health, Science and Technology 2016; 14(2): 13-18.
15. Pripdeevech P and Saansoomchai J. Antibacterial activity and chemical composition of essential oil and various extracts of Fagraea fragrans Roxb. flowers. Chiang Mai Journal of Science 2013; 40(2): 214-223.
16. Jonville M, Baghdikian B, Ollivier E, Angenot L, Frederich M, Legault J. Anti-inflammatory potency of the traditionally used antimalarial plant Fagraea fragrans. Planta Medica 2016; 76(12): WSI_6.
17. Jonville MC, Capel M, Frédérich M, Angenot L, Dive G, Faure R, Azas N, Ollivier E. Fagraldehyde, a secoiridoid isolated from Fagraea fragrans. Journal of natural products 2008; 71(12): 2038-2040.
18. Nguyen-Pouplin J, Tran H, Tran H, Phan TA, Dolecek C, Farrar J, Grellier P. Antimalarial and cytotoxic activities of ethnopharmacologically selected medicinal plants from South Vietnam. Journal of Ethnopharmacology 2007; 109(3): 417-427.
19. Madmanang S, Cheyeng N, Heembenmad S, Mahabusarakam W, Saising J, Seeger M, Chakthong S. Constituents of Fagraea fragrans with antimycobacterial activity in combination with erythromycin. Journal of natural products 2016; 79(4): 767-774.
20. Chomcheon P, Sriubolmas N, Wiyakrutta S, Ngamrojanavanich N, Chaichit N, Mahidol C, Ruchirawat S, Kittakoop P. Cyclopentenones, scaffolds for organic syntheses produced by the endophytic fungus mitosporic dothideomycete sp. LRUB20. Journal of natural products 2006; 69(9):1351-1353.
21. Reissinger A, Vilich V, & Sikora RA. Detection of fungi in planta: effectiveness of surface sterilization methods. Mycological Research, 2001; 105(5), 563-566.
22. Paterson RRM and Bridge PD. Biochemical techniques for filamentous fungi. Oxon, UK: CAB International; 1994.
23. Sriubolmas N, Tung A., Sawatchupong R., Ruangrungsi N., and Wiyakrutta S. Antimicrobial activities of endophytic fungi isolated from selected Thai medicinal plants. p. 228-237. In Hedreyda CT, and Zabat MEA (eds.), Proceeding of the 4th Asia-Pacific Biotechnology Congress & 30th Annual Philippine Society for Microbiology, Inc.; Convention, Cebu City (Philippines). May 16-18, 2001.
24. Sutton BC. The Coelomycetes. Surrey, England: Commonwealth Mycological Institute. 1980.
Published
2020-06-29
How to Cite
1.
Insain P, Boonsom T, Pharadai K, Sriubolmas N. Antimicrobial Activity of Endophytic Fungi Isolated From Fagraea fragrans Roxb. HCUJOURNAL [Internet]. 2020Jun.29 [cited 2020Aug.13];24(1):21-2. Available from: https://he01.tci-thaijo.org/index.php/HCUJOURNAL/article/view/187705
Section
Research article