D08. Ultrasonic Assisted Extraction Enhanced Total Phenolic and Antioxidant Activities from Aegle marmelos (L.) Corr. Extract
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Abstract
Introduction: The ultrasonic assisted extraction (UAE) is a green technology to extract bioactive compounds from natural sources. The extraction process showed the high efficiency in part of activity and reducing time. Aegle marmelos (L.) Corr. or bael has been used as traditional medicine to dysentery, fever, diabetes, asthma, heart problems. There are various kinds of secondary metabolites have been reports and their pharmaceutical activities.
Objective: To determine total phenolic content and antioxidant activities of two methods of extraction; maceration and ultrasonic assisted extraction.
Methods: The powder of A. marmelos was extract by maceration and UAE using water and EtOH as solvents. The extract was investigated total phenolic content and antioxidant activity including DPPH and FRAP assays.
Results: The ultrasonic assisted extraction of ethanolic extract at 15 minutes showed the highest values of total phenolic content at 79.75±0.05 mg GAE/g crude extract. Moreover, the antioxidant activity of extract using UAE at 30 minutes exhibited the most percent inhibition about 39.50% in DPPH assay whereas hot water extraction found the better antioxidant activity in FRAP assay with the value of 355.75 μM/g crude extract.
Conclusion: UAE method was high efficiency in the extraction especially in term of extraction time and bioactivity.
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