D06. The Protective Effects of Marine Microalgae Extract on Skin Cells-inducted Sun Ray
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Abstract
Introduction: In the present study, two kinds of marine microalgae, Tisochrysis lutea (Coccolithophyceae) is a single microalga widely used as live food in aquaculture. Tetraspora gelatinosa (Tetrasporaceae) is spherical to elliptical cell, arranged two-by-two or four-by-four, with 2 pseudoflagella extending beyond the mucilage. They are able to produce high value chemicals which can be applied in feed and food, pharmaceutical, nutraceutical and cosmeceutical industries.
Objective: To determine effects of T.lutea and T. gelatinosa extracts on skin cells functions.
Methods: Dried microalgal biomass of microalgae were extract with ethanol. The biological activity of microalgae extracts was investigated on skin cells such as keratinocyte cells (HaCaT) and melanoma cells (B16F10). The protective effect against photo-inducted inflammation, oxidative stress and melanogenesis were investigated. Cytotoxicity of microalgae extracts were determined using WST-assay. Sun ray was treatment to HaCaT cells for inflammation and oxidative stress effect. and B16F10 cells was treated sun ray to inducing and melanin production.
Results: The content of the biochemical components in two marine microalgae showed total carbohydrate and protein content were the main component of T. gelatinosa. The effect of T. lutea and T. gelatinosa were exhibited to suppressing cytokine secretion, melanin content, and ROS production on sun ray-induced skin cells. Moreover, T. gelatinosa was showed significant protective effect on ROS scavenging.
Conclusion: T. lutea and T. gelatinosa are marine microalgae that have benefit on skin cells to protect sun ray induce cell damage and skin pigmentation. The protection activity on keratinocyte cells including anti-inflammation via cytokine PGE2 suppressing and anti-oxidative by ROS scavenging from sun-ray stimulation. Therefore, both of marine microalgae may have the potential to use as an active ingredient in cosmetic or cosmeceutical products.
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